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PMID: 3773052 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human respiratory syncytial virus glycoprotein G expressed from a recombinant vaccinia virus vector protects mice against live-virus challenge.

Journal of virology ·Vol. 60 ·No. 2 ·1986-11-00 ·Pages 607-13

Stott EJ, Ball LA, Young KK, Furze J, Wertz GW

Abstract

Recombinant vaccinia virus vectors were constructed which expressed the major surface glycoprotein G of human respiratory syncytial (RS) virus. The biological activity of the G protein expressed from these vectors was assayed. Inoculation of rabbits with live recombinant virus induced high titers of antibody which specifically immunoprecipitated RS virus G protein and was capable of neutralizing RS virus infectivity. Immunization of mice by either the intranasal or the intraperitoneal route with recombinant virus that expressed only the G protein resulted in complete protection of the lower respiratory tract upon subsequent challenge with live RS virus.

MeSH Terms
Animals Antibodies, Viral/biosynthesis DNA, Recombinant Genetic Vectors Humans Immunization Membrane Glycoproteins Mice Neutralization Tests Rabbits Respiratory Syncytial Viruses/genetics,immunology Respirovirus Infections/prevention & control Vaccinia virus/genetics,immunology Viral Envelope Proteins Viral Proteins/genetics,immunology
Chemicals
Antibodies, Viral DNA, Recombinant G protein, vesicular stomatitis virus Membrane Glycoproteins Viral Envelope Proteins Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stott E J
Ball L A
Young K K
Furze J
Wertz G W
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33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.2.607-613.1986
Published
1986-11-00
Pages
607-13
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288932
Subset
IM
Grants
NIAID NIH HHS · AI12464 · United States
NIAID NIH HHS · AI18270 · United States
NIAID NIH HHS · AI20181 · United States
External Links
PubMed source DOI: 10.1128/JVI.60.2.607-613.1986 PMC Full Text View journal details
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