Human antibody-dependent cell-mediated cytotoxicity against target cells infected with respiratory syncytial virus.

Clinical and experimental immunology ·Vol. 28 ·No. 1 ·1977-04-00 ·Pages 19-26

Scott R, de Landazuri MO, Gardner PS, Owen JJ

Abstract

A chromium release assay was established to study human antibody-dependent cell-mediated cytotoxicity (ADCC) of HEp 2 cells infected with respiratory syncytial (RS) virus. Human peripheral blood lymphocytes in the presence of specific antibody to RS virus caused in vitro lysis of RS virus infected target cells. ADCC was detected in sera of infants with RS virus infections and in specimens of colostrum. The ability of serum or colostrum to mediate the cytotoxic reaction appeared to be related to the level of specific IgG, or IgA antibody to RS virus, as detected by membrane fluorescence. Separation of effector cells by their glass adherence properties showed that the ability to produce cytotoxicity resided in non-adherent effector cells.

MeSH Terms

Antibodies, Viral Antigens, Viral Cells, Cultured Colostrum/immunology Cytotoxicity Tests, Immunologic Humans Immunity, Cellular Infant Lymphocytes/immunology Respiratory Syncytial Viruses/immunology Respiratory Tract Infections/immunology

Chemicals

Antibodies, Viral Antigens, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Scott R

de Landazuri M O

Gardner P S

Owen J J

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24 references, click to expand

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Article Info

Journal

Clinical and experimental immunology

Abbr.

Clin Exp Immunol

ISSN

0009-9104

Published

1977-04-00

Pages

19-26

Language

English

Region

England

NLM ID

0057202

PMCID

PMC1540874

Subset

External Links

PubMed source PMC Full Text View journal details

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Human antibody-dependent cell-mediated cytotoxicity against target cells infected with respiratory syncytial virus.

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