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PMID: 3694174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Passive properties and membrane currents of canine ventricular myocytes.

The Journal of general physiology ·Vol. 90 ·No. 5 ·1987-11-00 ·Pages 671-701

Tseng GN, Robinson RB, Hoffman BF

Abstract

The membrane potential and membrane currents of single canine ventricular myocytes were studied using either single microelectrodes or suction pipettes. The myocytes displayed passive membrane properties and an action potential configuration similar to those described for multicellular dog ventricular tissue. As for other cardiac cells, in canine ventricular myocytes: (a) an inward rectifier current plays an important role in determining the resting membrane potential and repolarization rate; (b) a tetrodotoxin-sensitive Na current helps maintain the action potential plateau; and (c) the Ca current has fast kinetics and a large amplitude. Unexpected findings were the following: (a) in approximately half of the myocytes, there is a transient outward current composed of two components, one blocked by 4-aminopyridine and the other by Mn or caffeine; (b) there is clearly a time-dependent outward current (delayed rectifier current) that contributes to repolarization; and (c) the relationship of maximum upstroke velocity of phase 0 to membrane potential is more positive and steeper than that observed in cardiac tissues from Purkinje fibers.

MeSH Terms
Animals Dogs Electrophysiology Heart/physiology In Vitro Techniques Membrane Potentials Myocardium/cytology Ventricular Function
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tseng G N
Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Robinson R B
Hoffman B F
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.90.5.671
Published
1987-11-00
Pages
671-701
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2228877
Subset
IM
Grants
NHLBI NIH HHS · HL-30557 · United States
External Links
PubMed source DOI: 10.1085/jgp.90.5.671 PMC Full Text View journal details
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