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PMID: 3684600 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Methyl transferases induced during chemical adaptation of M. luteus.

Nucleic acids research ·Vol. 15 ·No. 22 ·1987-11-25 ·Pages 9471-86

Riazuddin S, Athar A, Sohail A

Abstract

Three peaks of methyltransferase activity specific for MNNG alkylated DNA have been identified from extracts of chemically adapted M. luteus. They are designated as TI to TIII in order to their elution from a Sephadex G-75 column. The first one of these peaks has been purified to homogeneity. TI, is an inducible, unusually salt resistant, heat labile protein which corrects O6-methylguanine in alkylated DNA by the transfer of the O6-alkyl group to a cysteine amino acid in the TI protein. There is a stoichiometric relationship between the loss of O6-methylguanine from the DNA and the production of S-methylcysteine. Partially purified TII & TIII proteins show specificity for O4-alkylthymine and methyl phosphotriesters respectively. The mode of repair by the isolated methyltransferases is similar yet there is no competition for substrate specificity. The apparent molecular weights of TI, TII & TIII proteins are 31Kd, 22Kd, and 13Kd respectively.

MeSH Terms

Alkylating Agents/pharmacology Chloramphenicol/pharmacology Enzyme Induction Methylation Methylnitronitrosoguanidine/metabolism Methyltransferases/biosynthesis,isolation & purification Micrococcus/drug effects,enzymology Molecular Weight O(6)-Methylguanine-DNA Methyltransferase

Chemicals

Alkylating Agents Methylnitronitrosoguanidine Chloramphenicol Methyltransferases O(6)-Methylguanine-DNA Methyltransferase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Riazuddin S

Centre for Advanced Molecular Biology, University of the Punjab, Lahore, Pakistan.

Athar A

Sohail A

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25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/15.22.9471

Published

1987-11-25

Pages

9471-86

Language

English

Region

England

NLM ID

0411011

PMCID

PMC306481

Subset

External Links

PubMed source DOI: 10.1093/nar/15.22.9471 PMC Full Text View journal details

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