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PMID: 3903661 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stereospecific removal of methyl phosphotriesters from DNA by an Escherichia coli ada+ extract.

Nucleic acids research ·Vol. 13 ·No. 19 ·1985-10-11 ·Pages 7067-77

Weinfeld M, Drake AF, Saunders JK, Paterson MC

Abstract

The ada+ gene product, a DNA methyltransferase present in extracts from an Escherichia coli strain constitutive for the adaptive response, removes only half of the methyl phosphotriesters from alkylated DNA. Since DNA phosphotriesters occur in two isomeric configurations (denoted Rp and Sp), we examined whether this reflects a stereospecific mode of repair by the methyltransferase. Analysis by reverse-phase HPLC, phosphorus NMR and circular dichroism established that only triesters in the Sp configuration are acted upon by the E. coli extract.

MeSH Terms
Alkylating Agents/metabolism Circular Dichroism DNA Repair Escherichia coli/enzymology,genetics Methylation Methyltransferases/metabolism Models, Molecular Nucleic Acid Conformation Substrate Specificity Temperature
Chemicals
Alkylating Agents Methyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weinfeld M
Drake A F
Saunders J K
Paterson M C
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.19.7067
Published
1985-10-11
Pages
7067-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322023
Subset
IM
Grants
NCI NIH HHS · N01-CP-21029 · United States
External Links
PubMed source DOI: 10.1093/nar/13.19.7067 PMC Full Text View journal details
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