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PMID: 3662826 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cell type specific expression of pre S 1 antigen and secretion of hepatitis B virus surface antigen. Brief Report.

Archives of virology ·Vol. 96 ·No. 3-4 ·1987-00-00 ·Pages 249-56

Marquardt O, Heermann KH, Seifer M, Gerlich WH

Abstract

Production of the three hepatitis B surface (HBs) proteins was studied in a hepatoma cell line (PLC/PRF/5) and two HBs antigen secreting cell lines (HeLa and mouse L-cells), which had been transfected by a viral genome isolated by molecular cloning from PLC/PRF/5 chromosomal DNA. The DNA used for transfection contains the HBs-specific promoters and the enhancer which regulate the expression of HBs genes in the transfected cell lines. All three cell lines expressed well the small and middle HBs protein, but the larger pre S 1 containing protein was barely detectable in the L-cell. In vivo growth of the transfected HeLa cell as nude mouse tumour increased pre S 1 expression and suppressed secretion of HBsAg.

MeSH Terms
Animals Cell Line HeLa Cells/analysis,metabolism Hepatitis B Surface Antigens/analysis,biosynthesis,metabolism Humans Intracellular Fluid/analysis L Cells/analysis,metabolism Liver Neoplasms, Experimental/analysis,metabolism Mice Mice, Nude Tumor Cells, Cultured/analysis,metabolism
Chemicals
Hepatitis B Surface Antigens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marquardt O
Max-Planck-Institute of Biochemistry, Martinsried, Federal Republic of Germany.
Heermann K H
Seifer M
Gerlich W H
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01320964
Published
1987-00-00
Pages
249-56
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01320964 View journal details
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