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PMID: 3649292 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe strictly requires an intron-contained, conserved sequence element.

The EMBO journal ·Vol. 6 ·No. 6 ·1987-06-00 ·Pages 1757-63

Mertins P, Gallwitz D

Abstract

It has recently been argued that pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe may be more similar to splicing in metazoan species than in the budding yeast Saccharomyces cerevisiae. In this report we show that, contrary to this assumption, the conserved sequence element 5'-CTPu APy-3' found in all S. pombe introns 6-18 nucleotides upstream of the 3' splice site is, like the TACTAAC box in S. cerevisiae, indispensable for efficient splicing. The conserved adenine residue of this sequence is used for branch formation and point mutations introduced into the CTPuAPy sequence abolish splicing and seem not to result in the recruitment of cryptic branch sites. We also show that an S. cerevisiae intron is correctly excised in S. pombe whereby the TACTAAC box is used in branch formation.

MeSH Terms
Base Sequence Cell Nucleus/physiology Codon Genes, Fungal Introns Mutation Nucleic Acid Precursors/genetics RNA Precursors RNA Splicing RNA, Messenger/genetics Saccharomycetales/genetics Schizosaccharomyces/genetics
Chemicals
Codon Nucleic Acid Precursors RNA Precursors RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mertins P
Gallwitz D
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41 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-06-00
Pages
1757-63
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553552
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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