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PMID: 364489 Published · ppublish English Comparative Study Journal Article

Identification of insulin in rat brain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 11 ·1978-11-00 ·Pages 5737-41

Havrankova J, Schmechel D, Roth J, Brownstein M

Abstract

Insulin concentrations in acid/ethanol extracts of the whole rat brain were on the average 25 times higher than plasma insulin levels. Brain insulin was indistinguishable from authentic pancreatic insulin, based on its behavior in radioimmunoassay, radioreceptor assay, and bioassay and its chromatographic pattern on Sephadex G-50 column chromatography. Insulin was found in all regions of the brain examined, but distribution was uneven. Some regions had insulin concentrations as much as 100 times higher than in plasma; levels at least 10 times higher were found in other regions. The role of insulin in the central nervous system is not clear at present but, because both insulin and insulin receptors are abundant in the central nervous system, an extensive physiological regulation of the central nervous system by insulin is proposed.

MeSH Terms
Adipose Tissue/drug effects,metabolism Animals Biological Assay Brain/metabolism Brain Chemistry Glucose/metabolism Insulin/analysis,metabolism,pharmacology Islets of Langerhans/analysis Male Radioimmunoassay Rats Receptor, Insulin/metabolism
Chemicals
Insulin Receptor, Insulin Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Havrankova J
Schmechel D
Roth J
Brownstein M
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.11.5737
Published
1978-11-00
Pages
5737-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393044
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.11.5737 PMC Full Text View journal details
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