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PMID: 361575 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Phagocytosis of Cryptococcus neoformans by normal and thioglycolate-activated macrophages.

Infection and immunity ·Vol. 21 ·No. 3 ·1978-09-00 ·Pages 714-20

Swenson FJ, Kozel TR

Abstract

Phagocytosis of Cryptococcus neoformans by normal and thioglycolate-activated mouse peritoneal macrophages was studied. Thioglycolate-activated macrophages exhibited a lower percent phagocytosis than did normal macrophages. Differences in phagocytosis could not be attributed to differences in macrophage viability, minor variations in the concentration of adherent macrophages, or a general depression in activated macrophage phagocytosis. Thioglycolate-activated macrophages required heat-labile opsonins for optimal phagocytosis of non-encapsulated cryptococci, whereas nonactivated macrophages did not require heat-labile opsonins for phagocytosis of the yeast. Both types of macrophages exhibited similar sensitivity to the phagocytosis-inhibiting properties of cryptococcal polysaccharide. The results show that depletion of heat-labile opsonins from serum or inactivation of yeast-bound, heat-labile opsonins by polysaccharide cannot account for the phagocytosis-inhibiting properties of cryptococcal polysaccharide.

MeSH Terms
Animals Cryptococcus/physiology Cryptococcus neoformans/physiology Macrophages/drug effects,physiology Mice Opsonin Proteins Phagocytosis/drug effects Polysaccharides/pharmacology Thioglycolates/pharmacology
Chemicals
Opsonin Proteins Polysaccharides Thioglycolates
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Swenson F J
Kozel T R
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18 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.21.3.714-720.1978
Published
1978-09-00
Pages
714-20
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC422057
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.21.3.714-720.1978 PMC Full Text View journal details
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