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PMID: 170358 Published · ppublish English Journal Article

Differential stimulation of murine lymphoma growth in vitro by normal and BCG-activated macrophages.

The Journal of experimental medicine ·Vol. 142 ·No. 4 ·1975-10-01 ·Pages 887-902

Nathan CF, Terry WD

Abstract

Peritoneal macrophages from mice infected with Bacille Calmette-Guérin (BCG) and from normal mice were examined for their effects in vitro on thymidine uptake by 10 murine lymphomas, a murine fibroblast line, and a guinea pig hepatoma. Only the murine fibroblast line showed growth inhibition in the presence of BCG macrophages. For the majority of tumors, normal macrophages were profoundly stimulatory to tumor cell DNA synthesis, while BCG macrophages were much less stimulatory, without being frankly inhibitory. The effect of 2-mercaptoethanol on tumor cell growth was also studied. All lymphomas stimulated to grow more rapidly in vitro by normal macrophages were stimulated to a similar degree by 2-mercaptoethanol.

MeSH Terms
Animals BCG Vaccine Carcinoma, Hepatocellular/immunology Cells, Cultured DNA, Neoplasm/biosynthesis Female In Vitro Techniques Leukemia, Experimental/immunology Liver Neoplasms/immunology Lymphoma/immunology Macrophages/immunology Mercaptoethanol/pharmacology Mice Mycobacterium bovis Neoplasms, Experimental/immunology Stimulation, Chemical
Chemicals
BCG Vaccine DNA, Neoplasm Mercaptoethanol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nathan C F
Terry W D
References (48)
48 references, click to expand
  1. Two biological activities regulating cell proliferation found in cultures of peritoneal exudate cells.
    Nature. 1975 Jan 31;253(5490):359-61 PMID: 1078603
  2. [Non specific active immunotherapy by B.C.G. in virus induced leukemia in E male G2 mice in isogenic receiptients].
    Rev Fr Etud Clin Biol. 1967 Nov;12(9):912-4 PMID: 5591341
  3. Activation of human blood monocytes by products of sensitized lymphocytes.
    J Clin Invest. 1974 Feb;53(2):559-64 PMID: 11344570
  4. Allogeneic recognition and killing capacity of immune macrophages in mixed macrophage cultures (MMC).
    Cell Immunol. 1975 Feb;15(2):419-31 PMID: 122917
  5. Macrophage cytotoxicity in the mouse immune response against a skin allograft.
    J Immunol. 1975 Jan;114(1 Pt 1):195-9 PMID: 122985
  6. Inhibition of proliferation of lymphoma cells and T lymphocytes by suppressor cells from spleens of tumor-bearing mice.
    J Immunol. 1975 Jan;114(1 Pt 1):206-10 PMID: 122986
  7. Major changes in lymphocyte proliferation evoked by activated macrophages.
    Cell Immunol. 1975 Jun;17(2):542-51 PMID: 123831
  8. The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system.
    J Biol Chem. 1958 Jul;233(1):184-8 PMID: 13563465
  9. Effect of BCG infection on leukemia and polyoma in mice and hamsters.
    Proc Soc Exp Biol Med. 1962 Dec;111:739-42 PMID: 13929573
  10. HOMOGRAFT TARGET CELLS: SPECIFIC DESTRUCTION IN VITRO BY CONTACT INTERACTION WITH IMMUNE MACROPHAGES.
    Science. 1964 Sep 25;145(3639):1427-9 PMID: 14172631
  11. Macrophages activated in vitro with lymphocyte mediators kill neoplastic but not normal cells.
    J Immunol. 1975 Jan;114(1 Pt 2):293-9 PMID: 163856
  12. Possible role of macrophage mediated nonspecific cytotoxicity in tumour resistance.
    Nat New Biol. 1972 Jan 12;235(54):48-50 PMID: 4109405
  13. Immunoglobulin and theta-bearing murine leukemias and lymphomas.
    J Immunol. 1972 May;108(5):1146-51 PMID: 4112916
  14. Characterization of a lymphocyte factor which alters macrophage functions.
    J Exp Med. 1973 Feb 1;137(2):275-90 PMID: 4119587
  15. Macrophage cytotoxicity factor. A product of in vitro sensitized thymus-dependent cells.
    Eur J Immunol. 1973 Jan;3(1):56-8 PMID: 4197970
  16. The role of activated macrophages in specific and nonspecific cytostasis of tumor cells.
    J Immunol. 1974 Aug;113(2):507-16 PMID: 4210882
  17. Active immunotherapy of L1210 leukaemia applied after the graft of tumour cells.
    Br J Cancer. 1969 Dec;23(4):814-24 PMID: 4312660
  18. Receptors for immunoglobulin and complement on mouse leukemias and lymphomas.
    J Immunol. 1972 Feb;108(2):325-8 PMID: 4340285
  19. Immunocytology of cultured IgM-forming cells of mouse. I. Requirement of phagocytic cell factor for the growth of IgM-forming tumor cells in tissue culture.
    J Immunol. 1972 Dec;109(6):1193-200 PMID: 4343748
  20. The role of macrophages in the cytotoxic killing of tumour cells in vitro. I. Primary immunization of lymphocytes in vitro for target cell killing and the mechanism of lymphocyte-macrophage cooperation.
    Immunology. 1973 Oct;25(4):631-44 PMID: 4356674
  21. Selective cytotoxicity of peritoneal leucocytes for neoplastic cells.
    Cell Immunol. 1973 Dec;9(3):339-52 PMID: 4357184
  22. Heterogenous populations of cytotoxic cells in the peritoneal cavity of BALB/c mice immunized with allogeneic EL4 leukemia cells.
    Cell Immunol. 1974 May;12(2):280-9 PMID: 4458965
  23. Modulation of cell proliferation by macrophages: a possible function apart from cytotoxic tumour rejection.
    Br J Cancer. 1974 Nov;30(5):401-15 PMID: 4469193
  24. Discrimination between neoplastic and non-neoplastic cells in vitro by activated macrophages.
    J Natl Cancer Inst. 1974 Nov;53(5):1487-92 PMID: 4473563
  25. Immune responses in vitro. I. Culture conditions for antibody synthesis.
    Cell Immunol. 1972 Feb;3(2):264-76 PMID: 4551691
  26. Combined chemoimmunostimulation therapy against murine leukemia.
    Cancer Res. 1972 May;32(5):904-7 PMID: 4552917
  27. Mechanism of immunologically specific killing of tumour cells by macrophages.
    Nature. 1972 Mar 24;236(5343):168-70 PMID: 4553694
  28. The cytotoxic effect of mouse macrophages on syngeneic and allogeneic erythrocytes.
    J Exp Med. 1973 Mar 1;137(3):807-20 PMID: 4570019
  29. BCG and cancer.
    N Engl J Med. 1974 Jun 27;290(26):1458-69 PMID: 4598691
  30. Enhancement of the humoral response of T cell-depleted murine spleens by a factor derived from human monocytes in vitro.
    J Immunol. 1974 Sep;113(3):925-33 PMID: 4606516
  31. Correlation of increased metabolic activity, resistance to infection, enhanced phagocytosis, and inhibition of bacterial growth by macrophages from Listeria- and BCG-infected mice.
    Infect Immun. 1972 Apr;5(4):499-504 PMID: 4629124
  32. Metabolic and functonal studies on activated mouse macrophages.
    J Exp Med. 1973 Feb 1;137(2):537-42 PMID: 4631141
  33. Promotion of replication in lymphoid cells by specific thiols and disulfides in vitro. Effects on mouse lymphoma cells in comparison with splenic lymphocytes.
    J Exp Med. 1973 Sep 1;138(3):574-92 PMID: 4727914
  34. The effect of 2-mercaptoethanol on murine mixed lymphocyte cultures.
    J Exp Med. 1974 Apr 1;139(4):1025-30 PMID: 4816300
  35. In vitro inhibition of tumour cell growth and DNA synthesis by peritoneal and lung macrophages from mice injected with Corynebacterium parvum.
    Int J Cancer. 1974 Apr 15;13(4):478-88 PMID: 4841814
  36. Destruction of hamster ovarian cell cultures by peritoneal macrophages from mice treated with Corynebacterium granulosum.
    J Natl Cancer Inst. 1974 Jun;52(6):1839-42 PMID: 4857795
  37. Tumor cytotoxicity in vitro by macrophages from mice infected with mycobacterium bovis strain BCG.
    J Natl Cancer Inst. 1974 Jun;52(6):1887-95 PMID: 4857796
  38. The host response to Calmette-Guérin bacillus infection in mice.
    J Exp Med. 1969 May 1;129(5):1079-107 PMID: 4976109
  39. The in vitro induction and release of a cell toxin by immune C57B1-6 mouse peritoneal macrophages.
    Cell Immunol. 1972 Jan;3(1):88-100 PMID: 5009049
  40. Thymocytes from mice immunized against an allograft render bone-marrow cells specifically cytotoxic.
    J Exp Med. 1972 Jan;135(1):150-64 PMID: 5009704
  41. Response of syngeneic murine lymphomata to immunotherapy in relation to the antigenicity of the tumour.
    Br J Cancer. 1972 Jun;26(3):174-82 PMID: 5047141
  42. Control of carcinogenesis: a possible role for the activated macrophage.
    Science. 1972 Sep 15;177(4053):998-1000 PMID: 5055947
  43. Immune response restoration with macrophage culture supernatants.
    Science. 1971 Jun 4;172(3987):1047-8 PMID: 5103320
  44. Endotoxin and double stranded RNA render macrophages cytotoxic.
    Nat New Biol. 1971 Jul 21;232(29):76-8 PMID: 5285341
  45. Cooperation of immune lymphoid cells with macrophages in tumour immunity.
    Nature. 1970 Nov 14;228(5272):620-2 PMID: 5529055
  46. Cytochemical identification of monocytes and granulocytes.
    Am J Clin Pathol. 1971 Mar;55(3):283-90 PMID: 5549896
  47. Alterations of macrophage functions by mediators from lymphocytes.
    J Exp Med. 1971 Jun 1;133(6):1356-76 PMID: 5576335
  48. Role of macrophages in in vitro induction of T-helper cells.
    Nature. 1975 Mar 27;254(5498):352-4 PMID: 1090850
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.142.4.887
Published
1975-10-01
Pages
887-902
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189947
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.142.4.887 PMC Full Text View journal details
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