Home LiteratureArticle Details
PMID: 3549694 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58.

Journal of bacteriology ·Vol. 169 ·No. 4 ·1987-04-00 ·Pages 1529-36

Hirooka T, Rogowsky PM, Kado CI

Abstract

The virE locus that is responsible for the efficiency of infection by Agrobacterium tumefaciens (T. Hirooka and C. Kado, J. Bacteriol. 168:237-243, 1986) is located next to the right boundary of the virulence (Vir) region of the nopaline plasmid pTiC58. This locus is very similar to the virE locus of octopine type Ti plasmids on the basis of nucleotide and amino acid sequence comparisons as well as genetic complementation analyses. The nucleotide sequence of virE revealed three open reading frames, arranged as an operon, with a potential coding capacity for proteins of 9, 7.1, and 63.5 kilodaltons. The promoter region of virE was analyzed by using gene fusions to promoterless cat and lux genes. Two different promoters were detected, one which operates in A. tumefaciens and one which operates in Escherichia coli. virE is transcribed from left to right toward the T region. In A. tumefaciens, the expression of virE was induced by acetosyringone and required the presence of pTiC58.

MeSH Terms
Acetophenones/pharmacology Arginine/analogs & derivatives,biosynthesis Base Sequence Escherichia coli/genetics Genetic Complementation Test Operon Plant Tumors/microbiology Plasmids Promoter Regions, Genetic Rhizobium/genetics,metabolism Transcription, Genetic
Chemicals
Acetophenones nopaline acetosyringone Arginine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hirooka T
Rogowsky P M
Kado C I
References (27)
27 references, click to expand
  1. Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58.
    Plasmid. 1984 Sep;12(2):111-8 PMID: 6095350
  2. Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region.
    Mol Gen Genet. 1984;193(1):1-7 PMID: 6318043
  3. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  4. Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping.
    Plasmid. 1980 Mar;3(2):193-211 PMID: 6100893
  5. T-DNA of the Agrobacterium Ti and Ri plasmids.
    Annu Rev Genet. 1982;16:357-84 PMID: 6297376
  6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  7. Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.
    Gene. 1982 Dec;20(2):305-16 PMID: 6299895
  8. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  9. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.
    EMBO J. 1985 Apr;4(4):891-8 PMID: 2990912
  10. Isolation of a recombination deficient Agrobacterium tumefaciens mutant.
    Mol Gen Genet. 1979 Jun 7;173(2):171-5 PMID: 290851
  11. Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2669-73 PMID: 2986128
  12. Regulation of Ti plasmid virulence genes by a chromosomal locus of Agrobacterium tumefaciens.
    J Bacteriol. 1985 Nov;164(2):774-81 PMID: 4055699
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. Rapid procedure for detection and isolation of large and small plasmids.
    J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
  15. Tumor-inducing (Ti) plasmids of Agrobacterium share extensive regions of DNA homology.
    J Bacteriol. 1978 Dec;136(3):1178-83 PMID: 721776
  16. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  17. Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens.
    J Mol Biol. 1981 Oct 25;152(2):183-208 PMID: 6276566
  18. trans-Acting virulence functions of the octopine Ti plasmid from Agrobacterium tumefaciens.
    J Bacteriol. 1984 May;158(2):754-6 PMID: 6373732
  19. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.
    Cell. 1977 Jun;11(2):263-71 PMID: 890735
  20. A plant cell factor induces Agrobacterium tumefaciens vir gene expression.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):379-83 PMID: 16593648
  21. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  22. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  23. Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens.
    Mol Gen Genet. 1982;188(3):425-32 PMID: 6962315
  24. Location of the right boundary of the virulence region on Agrobacterium tumefaciens plasmid pTiC58 and a host-specifying gene next to the boundary.
    J Bacteriol. 1986 Oct;168(1):237-43 PMID: 3019998
  25. Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid.
    J Bacteriol. 1983 Feb;153(2):878-83 PMID: 6296058
  26. Units of genetic expression in the virulence region of a plant tumor-inducing plasmid of Agrobacterium tumefaciens.
    Mol Gen Genet. 1982;188(3):418-24 PMID: 6298572
  27. A comparison of virulence determinants in an octopine Ti plasmid, a nopaline Ti plasmid, and an Ri plasmid by complementation analysis of Agrobacterium tumefaciens mutants.
    Plasmid. 1984 May;11(3):195-205 PMID: 6087390
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.4.1529-1536.1987
Published
1987-04-00
Pages
1529-36
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211979
Subset
IM
Grants
NCI NIH HHS · CA-11526 · United States
Databases
GENBANK
M15814
External Links
PubMed source DOI: 10.1128/jb.169.4.1529-1536.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2