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PMID: 3545815 Published · ppublish English Journal Article

Complementation of sensitivity to alkylating agents in Escherichia coli and Chinese hamster ovary cells by expression of a cloned bacterial DNA repair gene.

The EMBO journal ·Vol. 5 ·No. 12 ·1986-12-01 ·Pages 3195-200

Kataoka H, Hall J, Karran P

Abstract

Dual expression vectors derived from pSV2gpt and encoding all or part of the Escherichia coli ada+ gene have been constructed. Following transformation into an E. coli ada strain or transfection and stable integration into the genome of Chinese hamster ovary (CHO) cells, plasmid vectors containing the whole ada+ gene conferred resistance to both killing and mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Thus, the bacterial DNA repair gene was functionally expressed in the mammalian cells. Plasmids containing an N-terminal fragment of the ada+ gene which encoded only one of the two methyltransferase activities of the Ada protein did not significantly protect E. coli or CHO cells against MNNG. These results are consistent with the central role of the intact ada+ gene in controlling the adaptive response to alkylating agents in E. coli. However, the data further suggest that some alkylation lesions in DNA, such as O6-methylguanine, may exert partly different biological effects in E. coli and mammalian cells.

MeSH Terms
Animals Cell Line Cricetinae Cricetulus DNA Glycosylases DNA Repair Escherichia coli/drug effects,genetics Female Genes, Bacterial/drug effects Genetic Complementation Test Kinetics Methyl Methanesulfonate/pharmacology Methylnitronitrosoguanidine/pharmacology Methyltransferases/genetics N-Glycosyl Hydrolases/genetics Ovary Plasmids
Chemicals
Methylnitronitrosoguanidine Methyl Methanesulfonate Methyltransferases DNA Glycosylases N-Glycosyl Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kataoka H
Hall J
Karran P
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40 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-12-01
Pages
3195-200
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167312
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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