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PMID: 3526337 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Suppression of human DNA alkylation-repair defects by Escherichia coli DNA-repair genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5607-10

Samson L, Derfler B, Waldstein EA

Abstract

The ada-alkB operon protects Escherichia coli against the effects of many alkylating agents. We have subcloned it into the pSV2 mammalian expression vector to yield pSV2ada-alkB, and this plasmid has been introduced into Mer- HeLa S3 cells, which are extremely sensitive to killing and induction of sister chromatid exchange by alkylating agents. One transformant (the S3-9 cell line) has several integrated copies of pSV2ada-alkB and was found to express a very high level of the ada gene product, the 39-kDa O6-methylguanine-DNA methyltransferase. S3-9 cells were found to have become resistant to killing and induction of sister chromatid exchange by two alkylating agents, N-methyl-N'-nitro-N-nitrosoguanidine and N,N'-bis(2-chloroethyl)-N-nitro-sourea. This shows that bacterial DNA alkylation-repair genes are able to suppress the alkylation-repair defects in human Mer- cells.

MeSH Terms
Alkylating Agents/toxicity Alkylation Cloning, Molecular DNA Repair Drug Resistance Escherichia coli/enzymology,genetics Genetic Vectors HeLa Cells Humans Methyltransferases/genetics O(6)-Methylguanine-DNA Methyltransferase Phenotype Species Specificity Suppression, Genetic
Chemicals
Alkylating Agents Methyltransferases O(6)-Methylguanine-DNA Methyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Samson L
Derfler B
Waldstein E A
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5607
Published
1986-08-00
Pages
5607-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386337
Subset
IM
Grants
NIEHS NIH HHS · 1-P01-ES03926 · United States
NCI NIH HHS · CA35895 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.15.5607 PMC Full Text View journal details
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