A monoclonal antibody with binding and inhibiting activity towards human pancreatic carcinoma cells. I. Immunohistological and immunochemical characterization of a murine monoclonal antibody selecting for well differentiated adenocarcinomas of the pancreas.

Cancer immunology, immunotherapy : CII ·Vol. 23 ·No. 3 ·1986-00-00 ·Pages 185-91

Bosslet K, Kern HF, Kanzy EJ, Steinstraesser A, Schwarz A, Lüben G, Schorlemmer HU, Sedlacek HH

Abstract

The murine monoclonal antibody (MAb) BW 494 was characterized in relation to its tissue specificity, the epitope recognized, in vitro and in vivo radiolocalization and its potential to mediate antibody dependent cellular cytotoxicity (ADCC) and complement mediated cytolysis (CMC). The MAb defined carbohydrate epitope located on a greater than 200 k daltons glycoprotein was mainly expressed on the majority of well differentiated adenocarcinomas of the pancreas. Furthermore, the epitope is accessible to MAb BW 494 in vivo, allowing an enrichment of radioactive antibody at the tumor site in nude mice. Additionally, MAb BW 494 is able to use human peripheral blood lymphocytes as effector cells for ADCC reactions against appropriate tumor target cells in vitro. In contrast, the antibody does not mediate human or rabbit CMC.

MeSH Terms

Adenocarcinoma/immunology,pathology Animals Antibodies, Monoclonal/immunology Antibodies, Neoplasm/immunology Antibody Specificity Antibody-Dependent Cell Cytotoxicity Antigens, Neoplasm/immunology Cell Differentiation Complement Activation Cytotoxicity, Immunologic Glycoproteins/immunology Humans Immunoenzyme Techniques Mice Molecular Weight Neoplasm Proteins/immunology Neoplasms, Experimental/diagnostic imaging Pancreatic Neoplasms/immunology,pathology Radionuclide Imaging Tissue Distribution

Chemicals

Antibodies, Monoclonal Antibodies, Neoplasm Antigens, Neoplasm Glycoproteins Neoplasm Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Bosslet K

Kern H F

Kanzy E J

Steinstraesser A

Schwarz A

Lüben G

Schorlemmer H U

Sedlacek H H

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Article Info

Journal

Cancer immunology, immunotherapy : CII

Abbr.

Cancer Immunol Immunother

ISSN

0340-7004

DOI

10.1007/BF00205648

Published

1986-00-00

Pages

185-91

Language

English

Region

Germany

NLM ID

8605732

Subset

External Links

PubMed source DOI: 10.1007/BF00205648 View journal details

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A monoclonal antibody with binding and inhibiting activity towards human pancreatic carcinoma cells. I. Immunohistological and immunochemical characterization of a murine monoclonal antibody selecting for well differentiated adenocarcinomas of the pancreas.

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