Nucleotide sequence of the tag gene from Escherichia coli.

Nucleic acids research ·Vol. 14 ·No. 9 ·1986-05-12 ·Pages 3763-72

Steinum AL, Seeberg E

Abstract

We have determined the complete nucleotide sequence of the tag gene, encoding 3-methyladenine DNA glycosylase I from Escherichia coli. From the nucleotide sequence it is deduced that the tag enzyme consists of 187 amino-acids and has a calculated molecular weight of 21.1 kdaltons. The tag enzyme is unusually rich in cysteine (8 residues) with a cluster of three consecutive cysteines near the C-terminal end. The tag coded DNA glycosylase does not show significant sequence homology to the alkA coded glycosylase in spite of that both of these enzymes catalyze the release of free 3-methyladenine from alkylated DNA.

MeSH Terms

Amino Acids/analysis Base Sequence Codon DNA Glycosylases DNA, Bacterial/genetics Escherichia coli/genetics Genes Genes, Bacterial N-Glycosyl Hydrolases/genetics Promoter Regions, Genetic Solubility Transcription, Genetic

Chemicals

Amino Acids Codon DNA, Bacterial 3-methyladenine-DNA glycosylase DNA Glycosylases N-Glycosyl Hydrolases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Steinum A L

Seeberg E

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29 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.9.3763

Published

1986-05-12

Pages

3763-72

Language

English

Region

England

NLM ID

0411011

PMCID

PMC339813

Subset

Databases

GENBANK

X03845

External Links

PubMed source DOI: 10.1093/nar/14.9.3763 PMC Full Text View journal details

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Nucleotide sequence of the tag gene from Escherichia coli.

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