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PMID: 6239774 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

3-Methyladenine residues in DNA induce the SOS function sfiA in Escherichia coli.

The EMBO journal ·Vol. 3 ·No. 11 ·1984-11-00 ·Pages 2569-73

Boiteux S, Huisman O, Laval J

Abstract

The induction by methylating agents of the SOS function sfiA was measured by means of a sfiA::lac operon fusion in Escherichia coli mutants defective in alkylation repair. The sfiA operon was turned on at a 10-fold lower concentration of methylmethane sulfonate or dimethyl sulfate in tagA strains, lacking specific 3-methyladenine-DNA glycosylase, than in wild-type strains. In contrast, the induction of sfiA by u.v. light was not affected by a tagA mutation. We confirm that tagA strains specifically accumulate 3-methyladenine in their DNA. We conclude that the persistence of 3-methyladenine in E. coli DNA most likely induces the SOS functions. Results on in vitro DNA synthesis further suggest that this induction is due to an unscheduled arrest of DNA synthesis at this lesion.

MeSH Terms
Adenine/analogs & derivatives,analysis Alkylating Agents/pharmacology Bacteriophage lambda/genetics DNA Glycosylases DNA Repair DNA, Bacterial/genetics Escherichia coli/drug effects,genetics Genes, Bacterial Genotype N-Glycosyl Hydrolases/metabolism Species Specificity
Chemicals
Alkylating Agents DNA, Bacterial 3-methyladenine 3-methyladenine-DNA glycosylase DNA Glycosylases N-Glycosyl Hydrolases Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Boiteux S
Huisman O
Laval J
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-11-00
Pages
2569-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557731
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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