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PMID: 3515321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequences of the E. coli uvrB gene and protein.

Nucleic acids research ·Vol. 14 ·No. 6 ·1986-03-25 ·Pages 2637-50

Arikan E, Kulkarni MS, Thomas DC, Sancar A

Abstract

The UvrB protein is one of the three subunits of the E. coli ABC excinuclease. We have reported the sequences of the other two subunits, the UvrA and UvrC proteins. In this paper the sequence of the UvrB protein is presented. The protein sequence was determined from the DNA sequence of the uvrB gene and was confirmed by sequencing the NH2-terminus of the UvrB protein and analyzing its overall amino acid composition. The coding region of uvrB is 2019 basepairs, specifying a protein of 672 amino acids and Mr of 76,118. The sequence of the UvrB protein shows a moderate level of homology to that of the UvrC protein and to the ATP binding site of the UvrA protein. During purification of UvrB protein a proteolytic product, UvrB, is produced in high quantities. We find that UvrB results from removal of about 40 amino acids from the COOH-terminus of the UvrB protein. The uvrB gene has complex regulatory features. On the 5' side, the coding region is preceded by 3 promoters, a DnaA box and an SOS box. On the 3' side the gene is followed by an REP (Repetitive Extragenic Palindrome) sequence which has been implicated in gene regulation by an unknown mechanism.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence Codon Endodeoxyribonucleases/genetics Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation Genes, Bacterial Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Serine Endopeptidases
Chemicals
Bacterial Proteins Codon Escherichia coli Proteins LexA protein, Bacteria Endodeoxyribonucleases endodeoxyribonuclease uvrABC Serine Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Arikan E
Kulkarni M S
Thomas D C
Sancar A
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.6.2637
Published
1986-03-25
Pages
2637-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339688
Subset
IM
Grants
NIGMS NIH HHS · GM32833 · United States
External Links
PubMed source DOI: 10.1093/nar/14.6.2637 PMC Full Text View journal details
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