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PMID: 3501370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The structure of cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion.

The EMBO journal ·Vol. 6 ·No. 12 ·1987-12-01 ·Pages 3647-53

Ringwald M, Schuh R, Vestweber D, Eistetter H, Lottspeich F, Engel J, Dölz R, Jähnig F, Epplen J, Mayer S

Abstract

We have determined the amino acid sequence of the Ca2+-dependent cell adhesion molecule uvomorulin as it appears on the cell surface. The extracellular part of the molecule exhibits three internally repeated domains of 112 residues which are most likely generated by gene duplication. Each of the repeated domains contains two highly conserved units which could represent putative Ca2+-binding sites. Secondary structure predictions suggest that the putative Ca2+-binding units are located in external loops at the surface of the protein. The protein sequence exhibits a single membrane-spanning region and a cytoplasmic domain. Sequence comparison reveals extensive homology to the chicken L-CAM. Both uvomorulin and L-CAM are identical in 65% of their entire amino acid sequence suggesting a common origin for both CAMs.

MeSH Terms
Amino Acid Sequence Animals Antigens, Surface Base Sequence Cadherins Calcium/pharmacology Calcium-Binding Proteins/genetics Cell Adhesion/drug effects Cell Adhesion Molecules Cell Line Genes Membrane Glycoproteins/genetics,physiology Mice Molecular Sequence Data Nucleic Acid Hybridization Teratoma
Chemicals
Antigens, Surface Cadherins Calcium-Binding Proteins Cell Adhesion Molecules Membrane Glycoproteins Calcium
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Ringwald M
Friedrich-Miescher-Laboratorium, der Max-Planck-Gesellschaft, Tübingen, FRG.
Schuh R
Vestweber D
Eistetter H
Lottspeich F
Engel J
Dölz R
Jähnig F
Epplen J
Mayer S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-12-01
Pages
3647-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553833
Subset
IM
Databases
GENBANK
X06339
External Links
PubMed source PMC Full Text View journal details
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