Identification of a putative cell adhesion domain of uvomorulin.

The EMBO journal ·Vol. 4 ·No. 13A ·1985-12-16 ·Pages 3393-8

Vestweber D, Kemler R

Abstract

A rat monoclonal antibody (DECMA-1) selected against the murine cell adhesion molecule uvomorulin blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. However, decompacted embryos eventually become recompacted in the presence of DECMA-1 and form blastocysts composed of both trophectoderm and inner cell mass. DECMA-1 also disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. DECMA-1 recognizes uvomorulin in extracts from mouse and dog tissues. Protease digestion of mouse and dog uvomorulin generated core fragments including one of 26 kd which reacted with DECMA-1. The same 26-kd fragment is recognized by anti-uvomorulin monoclonal antibodies which have been obtained from other laboratories and which dissociate MDCK cell monolayers and block the formation of the epithelial occluding barrier. This 26-kd fragment therefore seems to be involved in the adhesive function of uvomorulin.

MeSH Terms

Animals Antibodies, Monoclonal Antibody Specificity Antigens, Surface/immunology,physiology Cadherins Cell Adhesion Cell Adhesion Molecules Cells, Cultured Cleavage Stage, Ovum/cytology Dogs Epithelial Cells Epithelium/immunology Epitopes Fluorescent Antibody Technique Glycoproteins/immunology,physiology Mice Morula/cytology Peptide Fragments/immunology,physiology

Chemicals

Antibodies, Monoclonal Antigens, Surface Cadherins Cell Adhesion Molecules Epitopes Glycoproteins Peptide Fragments

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Vestweber D

Kemler R

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22 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1985-12-16

Pages

3393-8

Language

English

Region

England

NLM ID

8208664

PMCID

PMC554675

Subset

External Links

PubMed source PMC Full Text View journal details

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Identification of a putative cell adhesion domain of uvomorulin.

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