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PMID: 3472229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Natural UAG suppressor glutamine tRNA is elevated in mouse cells infected with Moloney murine leukemia virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 9 ·1987-05-00 ·Pages 2668-72

Kuchino Y, Beier H, Akita N, Nishimura S

Abstract

Two species of glutamine tRNA were isolated from mouse liver and their nucleotide sequences were determined. The minor glutamine tRNA(tRNA(UmUGGln)) that possesses UmUG (where Um stands for 2'-O-methyluridine) as the anticodon sequence was found to have suppressor activity for the UAG termination codon of tobacco mosaic virus RNA in a rabbit reticulocyte in vitro translation system. The amount of this suppressor glutamine tRNA in mouse liver was 1-2% of the amount of the major glutamine tRNA(tRNA(CUGGln)) that has the CUG anticodon sequence, but it was markedly increased in NIH 3T3 cells infected with Moloney murine leukemia virus and in Ehrlich ascites cells. These results support the hypothesis that tRNA(UmUGGln) actually functions in vivo as a suppressor tRNA that recognizes the UAG termination codon located at the gag-pol gene junction of Moloney murine leukemia virus and results in the synthesis of the virus-encoded protease.

MeSH Terms
Animals Base Sequence Carcinoma, Ehrlich Tumor/metabolism Cell Transformation, Neoplastic Cells, Cultured Liver/metabolism Mice Mice, Inbred Strains Moloney murine leukemia virus/genetics Nucleic Acid Conformation RNA, Transfer, Amino Acyl/genetics Suppression, Genetic Tobacco Mosaic Virus/genetics
Chemicals
RNA, Transfer, Amino Acyl
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuchino Y
Beier H
Akita N
Nishimura S
References (20)
20 references, click to expand
  1. Murine leukemia virus protease is encoded by the gag-pol gene and is synthesized through suppression of an amber termination codon.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1618-22 PMID: 3885215
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs with suppressor activity from tobacco plants.
    EMBO J. 1984 Feb;3(2):351-6 PMID: 16453503
  4. Fractionation of rat liver transfer ribonucleic acid. Isolation of tyrosine, valine, serine, and phenylalanine transfer ribonucleic acids and their coding properties.
    Biochemistry. 1969 Mar;8(3):832-42 PMID: 5781021
  5. Biological and structural differences between tRNAVal species isolated from rat ascites hepatoma cells and normal rat liver.
    J Biochem. 1981 Aug;90(2):535-44 PMID: 7028731
  6. Nucleotide sequence of a macronuclear DNA molecule coding for alpha-tubulin from the ciliate Stylonychia lemnae. Special codon usage: TAA is not a translation termination codon.
    Nucleic Acids Res. 1985 Jan 25;13(2):415-33 PMID: 2987795
  7. Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.
    Nucleic Acids Res. 1982 Oct 25;10(20):6421-32 PMID: 6924749
  8. Adenovirus stimulation of transcription by RNA polymerase III: evidence for an E1A-dependent increase in transcription factor IIIC concentration.
    EMBO J. 1986 Feb;5(2):343-54 PMID: 2940084
  9. Deviation from the universal code shown by the gene for surface protein 51A in Paramecium.
    Nature. 1985 Mar 14-20;314(6007):188-90 PMID: 3974722
  10. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  11. An unusual genetic code in nuclear genes of Tetrahymena.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2452-5 PMID: 3921962
  12. Does Paramecium primaurelia use a different genetic code in its macronucleus?
    Nature. 1985 Mar 14-20;314(6007):185-8 PMID: 3974721
  13. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.
    Biochim Biophys Acta. 1971 Feb 11;228(3):770-4 PMID: 5572614
  14. Transcription of class III genes: formation of preinitiation complexes.
    Science. 1983 Nov 18;222(4625):740-8 PMID: 6356356
  15. Identification of point mutations in the genome of the poliovirus Sabin vaccine LSc 2ab, and catalogue of RNase T1- and RNase A-resistant oligonucleotides of poliovirus type 1 (Mahoney) RNA.
    Virology. 1981 Jul 15;112(1):217-27 PMID: 6264681
  16. Nucleotide sequences of two aspartic acid tRNAs from rat liver and rat ascites hepatoma.
    J Biol Chem. 1981 Sep 10;256(17):9059-62 PMID: 6927846
  17. Partial suppression of an ochre mutation in Saccharomyces cerevisiae by multicopy plasmids containing a normal yeast tRNAGln gene.
    J Mol Biol. 1985 May 5;183(1):31-42 PMID: 2989539
  18. Tetrahymena thermophila glutamine tRNA and its gene that corresponds to UAA termination codon.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4758-62 PMID: 2991890
  19. Dramatic events in ciliate evolution: alteration of UAA and UAG termination codons to glutamine codons due to anticodon mutations in two Tetrahymena tRNAs.
    EMBO J. 1986 Jun;5(6):1307-11 PMID: 16453685
  20. The nucleotide sequence of a major glutamine tRNA from rat liver.
    Nucleic Acids Res. 1983 Apr 11;11(7):1991-6 PMID: 6403925
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.9.2668
Published
1987-05-00
Pages
2668-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304719
Subset
IM
Databases
GENBANK
M16251, M16252
External Links
PubMed source DOI: 10.1073/pnas.84.9.2668 PMC Full Text View journal details
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