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PMID: 6924749 Published · ppublish English Journal Article

Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.

Nucleic acids research ·Vol. 10 ·No. 20 ·1982-10-25 ·Pages 6421-32

Kuchino Y, Borek E, Grunberger D, Mushinski JF, Nishimura S

Abstract

Nucleotide sequences of normal mouse liver tRNAPhe and tumor-specific tRNAPhes isolated from Ehrlich ascites tumor and neuroblastoma cells were examined by post-labeling techniques. The results showed that their sequences are identical, except for changes in post-transcriptional modifications that are located in the anticodon region. Normal mouse liver tRNAPhe contained Cm32, Gm34 and YOH37. On the other hand, tumor-specific tRNAPhes were found in one of two possible configurations: 1) Cm32, Gm34 and Y*OH37 (under-modified YOH) or 2) C32, G34 and m1G37. The ratio of the two forms of tRNAPhes differed in different tumor cells; Ehrlich ascites tumor tRNAPhe had mainly Y*OH-containing tRNAPhe whereas neuroblastoma tRNAPhe has predominantly m1G-containing tRNAPhe. It was concluded that tumor-specific tRNAPhes are products of different extents of modification, rather than of new tRNA transcription.

MeSH Terms
Animals Anticodon/genetics Base Sequence Carcinoma, Ehrlich Tumor/genetics Guanine/analogs & derivatives,metabolism Liver/metabolism Mice Neoplasms, Experimental/genetics Neuroblastoma/genetics Nucleic Acid Conformation RNA Processing, Post-Transcriptional RNA, Transfer, Amino Acyl/genetics,isolation & purification
Chemicals
Anticodon RNA, Transfer, Amino Acyl wye Guanine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kuchino Y
Borek E
Grunberger D
Mushinski J F
Nishimura S
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.20.6421
Published
1982-10-25
Pages
6421-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326932
Subset
IM
Databases
GENBANK
K02684, K02685, K02686
External Links
PubMed source DOI: 10.1093/nar/10.20.6421 PMC Full Text View journal details
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