Isolation of hydroxy-Y base from rat liver tRNAPhe.

Nucleic acids research ·Vol. 6 ·No. 3 ·1979-03-00 ·Pages 993-9

Kasai H, Yamaizumi Z, Kuchino Y, Nishimura S

Abstract

A Y-base derivative was isolated from rat liver tRNAPhe and its structure was assigned to be alpha-(carboxyamino)-beta-hydroxy-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-a]purine-7-butyric acid dimethyl ester (hydroxy-Y), based on the results of mass spectrometry and chemical degradation. This modified base seems to be the major fluorescent component of rat liver tRNAPhe; the peroxy-Y base previously isolated from rat liver tRNAPhe and characterized by Nakanishi and his coworkers (1,2) was not present in our preparation.

MeSH Terms

Animals Chemical Phenomena Chemistry Liver/analysis Mass Spectrometry Phenylalanine Purinones/isolation & purification RNA, Transfer Rats

Chemicals

Purinones Phenylalanine hydroxy-Y base RNA, Transfer

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kasai H

Yamaizumi Z

Kuchino Y

Nishimura S

References (8)

8 references, click to expand

Tumour-specific phenylalanine tRNA contains two supernumerary methylated bases.
Nature. 1978 Jan 12;271(5641):126-9 PMID: 202873
Study on the properties and structure of the modified base Y+ of yeast tRNA Phe .
Biochim Biophys Acta. 1971 Jun 30;240(2):163-9 PMID: 4327043
Chemical modification of the fluorescent base in phenylalanine transfer ribonucleic acid.
Biochemistry. 1971 Jul 20;10(15):2969-76 PMID: 5000398
Structure of the "peroxy-Y base" from liver tRNA Phe .
Nat New Biol. 1971 Nov 24;234(47):107-9 PMID: 5289220
Isolation and structure determination of the fluorescent base from bovine liver phenylalanine transfer ribonucleic acid.
Biochemistry. 1973 Jan 16;12(2):188-93 PMID: 4566585
Isolation and characterization of peroxy-Y base from phenylalanine transfer ribonucleic acid of the plant, Lupinus luteus.
J Am Chem Soc. 1974 Dec 11;96(25):7797-80 PMID: 4464312
Structure of the fluorescent Y base from yeast phenylalanine transfer ribonucleic acid.
J Am Chem Soc. 1970 Dec 30;92(26):7617-9 PMID: 5490722
Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotides, nucleosides, and bases.
J Am Chem Soc. 1968 Jul 17;90(15):4182-4 PMID: 5655885

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.3.993

Published

1979-03-00

Pages

993-9

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327747

Subset

External Links

PubMed source DOI: 10.1093/nar/6.3.993 PMC Full Text View journal details

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Isolation of hydroxy-Y base from rat liver tRNAPhe.

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