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PMID: 3461454 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of cDNA clones encoding the entire B-26 region of human apolipoprotein B.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5678-82

Protter AA, Hardman DA, Sato KY, Schilling JW, Yamanaka M, Hort YJ, Hjerrild KA, Chen GC, Kane JP

Abstract

We report the characterization of intestine and liver cDNA clones for human apolipoprotein B (apoB) that map to the 5' end of the mRNA. The protein sequence encoded by the 5011 nucleotides derived from sequence analysis of these clones includes 1643 amino acid residues of the mature protein of Mr 184,000. The amino acid sequence at the amino terminus of B-74 peptide was determined and mapped to residue 1298. The size (Mr 145,700) and amino acid composition of the B-26 region encoded by these clones (including amino acid residues 1-1297) closely match the values obtained from the B-26 peptide. The amino acid sequence of peptide B-100 at the junction of peptides B-26 and B-74 (Phe-Lys decreases- Ser) shows structural homology to the site on human kininogen (Phe-Arg decreases- Ser) that is cleaved by the protease plasma kallikrein. The encoded protein contains five potential N-glycosylation sites and several regions in which the hydroxyamino acids, serine and threonine, are present in high abundance. The protein sequence presented in this report represents approximately 30% of the total B-100 protein and will aid in the characterization of additional cDNA clones.

MeSH Terms
Amino Acid Sequence Apolipoproteins B/genetics,metabolism Base Sequence Cloning, Molecular DNA/genetics Humans Intestines/physiology Liver/physiology Protein Conformation Receptors, LDL/metabolism
Chemicals
Apolipoproteins B Receptors, LDL DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Protter A A
Hardman D A
Sato K Y
Schilling J W
Yamanaka M
Hort Y J
Hjerrild K A
Chen G C
Kane J P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5678
Published
1986-08-00
Pages
5678-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386352
Subset
IM
Grants
NHLBI NIH HHS · HL 14237 · United States
Databases
GENBANK
M14081
External Links
PubMed source DOI: 10.1073/pnas.83.15.5678 PMC Full Text View journal details
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