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PMID: 3903660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human apolipoprotein B: identification of cDNA clones and characterization of mRNA.

Nucleic acids research ·Vol. 13 ·No. 19 ·1985-10-11 ·Pages 6937-53

Mehrabian M, Schumaker VN, Fareed GC, West R, Johnson DF, Kirchgessner T, Lin HC, Wang XB, Ma YH, Mendiaz E

Abstract

Apolipoprotein B (apoB) is a major protein component of low density and very low density lipoproteins. Because of its large size and heterogeneity, molecular studies of apoB have been difficult, and its structure and regulation remain poorly understood. We now report the identification of human apoB cDNA clones by antibody screening of hepatoma libraries in the expression vector lambda gt11. Both oligo(dT) primed and random primed libraries were constructed and screened with polyclonal antibodies to intact apoB, as well as with antibodies raised against a synthetic peptide based on the limited amino acid sequence available for apoB. The identity of the clones was unambiguously established by comparisons of the cloned cDNA sequences with apoB amino acid sequences. The clones hybridize to an exceptionally large 20 kb mRNA that is present in liver and intestine but not other tissues examined, consistent with the distribution expected from protein biosynthetic studies. The properties of the mRNA have implications for the biogenesis of the multiple apoB molecular weight forms secreted by liver and intestine.

MeSH Terms
Apolipoproteins B/genetics,immunology Cloning, Molecular DNA/genetics Gene Expression Regulation Humans Immunologic Techniques Intestines/physiology Liver/physiology Molecular Weight RNA, Messenger/genetics
Chemicals
Apolipoproteins B RNA, Messenger DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Mehrabian M
Schumaker V N
Fareed G C
West R
Johnson D F
Kirchgessner T
Lin H C
Wang X B
Ma Y H
Mendiaz E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.19.6937
Published
1985-10-11
Pages
6937-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322014
Subset
IM
Grants
NHLBI NIH HHS · HL27481 · United States
NHLBI NIH HHS · HLO7-386 · United States
Databases
GENBANK
X03045
External Links
PubMed source DOI: 10.1093/nar/13.19.6937 PMC Full Text View journal details
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