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PMID: 342509 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Promoter- and attenuator-related metabolic regulation of the Salmonella typhimurium histidine operon.

Journal of bacteriology ·Vol. 133 ·No. 2 ·1978-02-00 ·Pages 830-43

Winkler ME, Roth DJ, Hartman PE

Abstract

Expression of the histidine (his) operon in Salmonella typhimurium was found to be positively correlated with the intracellular level of guanosine tetraphosphate (ppGpp). Limitation for amino acids other than histidine elicited a histidine-independent metabolic regulation of the operon. In bacteria grown at decreased growth rates, his operon expression was metabolically regulated up to a point, after which further decreases in growth rate no longer resulted in further enhancement of operon expression. Studies using strains carrying various regulatory and deletion mutations indicated that metabolic regulation is achieved predominantly by increased RNA chain initiations at the primary (P1) and internal (P2) promoters. Metabolic regulation ordinarly did not involve changes in RNA chain terminations at the attenuator site of the his operon. A model is proposed that involves ppGpp-induced changes in RNA polymerase initiation specificity at particular promoters. A second, special form of metabolic regulation may operate which also is histidine independent, but does involve relief of attenuation.

MeSH Terms
Alanine/metabolism Carnosine/metabolism Chromosome Mapping Genes Glucose/metabolism Guanosine Tetraphosphate/metabolism Histidine/genetics,metabolism Operon Salmonella typhimurium/genetics,metabolism Serine/metabolism
Chemicals
Guanosine Tetraphosphate Serine Histidine Carnosine Glucose Alanine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Winkler M E
Roth D J
Hartman P E
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.2.830-843.1978
Published
1978-02-00
Pages
830-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222095
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.2.830-843.1978 PMC Full Text View journal details
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