Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants.

Journal of bacteriology ·Vol. 130 ·No. 1 ·1977-04-00 ·Pages 4-10

Bruni CB, Colantuoni V, Sbordone L, Cortese R, Blasi F

Abstract

Escherichia coli K-12 hisT mutants were isolated, and their properties were studied. These mutants are derepressed for the histidine operon, map close to the purF locus at about 49.5 min on the E. coli linkage map, and lack pseudouridylate synthetase activity. The defect in this enzyme leads to the absence of pseudouridines in the anticodon loop of several transfer ribonucleic acid species, as evidenced by the altered elution profile on reversed-phase chromatography and resistance to amino acid analogues. Finally, the hisT mutants studied have a reduced growth rate that appears to be linked to hisT, although it is not known whether it is due to the same mutation. The normal generation time can be restored by supplementing the medium with adenine, uracil, and isoleucine.

MeSH Terms

Adenine/metabolism Anticodon Chromosome Mapping Chromosomes, Bacterial Escherichia coli/enzymology,growth & development,metabolism Genes, Regulator Histidine/biosynthesis Hydro-Lyases Isoleucine/metabolism Mutation Operon Pentosyltransferases/metabolism Pseudouridine Uracil/metabolism Uridine Monophosphate

Chemicals

Anticodon Isoleucine Pseudouridine Histidine Uracil Uridine Monophosphate Pentosyltransferases Hydro-Lyases pseudouridylate synthetase Adenine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bruni C B

Colantuoni V

Sbordone L

Cortese R

Blasi F

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30 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.130.1.4-10.1977

Published

1977-04-00

Pages

4-10

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC235167

Subset

External Links

PubMed source DOI: 10.1128/jb.130.1.4-10.1977 PMC Full Text View journal details

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Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants.

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