Home LiteratureArticle Details
PMID: 3390164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Insulin-like growth factors 1 and 2 in bovine colostrum. Sequences and biological activities compared with those of a potent truncated form.

The Biochemical journal ·Vol. 251 ·No. 1 ·1988-04-01 ·Pages 95-103

Francis GL, Upton FM, Ballard FJ, McNeil KA, Wallace JC

Abstract

1. Insulin-like growth factors 1 and 2 (IGF-1 and IGF-2) together with a truncated form of IGF-1 were purified to homogeneity from bovine colostrum. 2. Two forms of IGF-1 were totally resolved from IGF-2 in the purification by h.p.l.c. involving cation-exchange and reverse-phase columns. 3. The complete amino acid sequences for all three forms of IGF were determined. The sequence of bovine IGF-1 was found to be identical with that of human IGF-1, and that of the variant lacked the N-terminal tripeptide Gly-Pro-Glu (-3N:IGF-1). Bovine IGF-2 was found to differ in three residues of the C-domain compared with human IGF-2, with serine, isoleucine and asparagine substituted for alanine, valine and serine respectively at positions 32, 35 and 36. 4. Protein synthesis in L6 rat myoblasts was stimulated and protein degradation inhibited in a co-ordinate response with all three IGFs. The relative potency in both processes was -3N:IGF-1 greater than IGF-1 greater than IGF-2. A similar order of potency was obtained for the stimulation of DNA synthesis by -3N:IGF-1 and IGF-1. The approximately 10-fold effect on biological activity of removing the N-terminal tripeptide is unexpected in view of current information on IGF-1 structure and function.

MeSH Terms
Amino Acid Sequence Animals Cattle Chromatography, High Pressure Liquid Chromatography, Ion Exchange Colostrum/analysis DNA/biosynthesis Female Insulin-Like Growth Factor I/isolation & purification Insulin-Like Growth Factor II/isolation & purification Molecular Sequence Data Peptide Fragments/pharmacology Protein Biosynthesis Somatomedins/isolation & purification Species Specificity
Chemicals
Peptide Fragments Somatomedins Insulin-Like Growth Factor I Insulin-Like Growth Factor II DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Francis G L
C.S.I.R.O. Division of Human Nutrition, Adelaide, South Australia.
Upton F M
Ballard F J
McNeil K A
Wallace J C
References (29)
29 references, click to expand
  1. Polypeptides with nonsuppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization, and some biological properties of forms I and II.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2365-9 PMID: 1065887
  2. Heterogeneity of insulin-like growth factor-I affinity for the insulin-like growth factor-II receptor: comparison of natural, synthetic and recombinant DNA-derived insulin-like growth factor-I.
    Biochem Biophys Res Commun. 1987 Feb 27;143(1):199-205 PMID: 2950860
  3. Primary structure of human insulin-like growth factor II.
    FEBS Lett. 1978 May 15;89(2):283-6 PMID: 658418
  4. Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II.
    J Biol Chem. 1981 Jul 10;256(13):6859-65 PMID: 7016879
  5. Isolation and partial sequence analysis of rat basic somatomedin.
    Endocrinology. 1982 Mar;110(3):734-40 PMID: 7056228
  6. Insulin-like growth factor I stimulates growth in hypophysectomized rats.
    Nature. 1982 Mar 18;296(5854):252-3 PMID: 7038514
  7. Synthesis and characterization of molecular hybrids of insulin and insulin-like growth factor I. The role of the A-chain extension peptide.
    J Biol Chem. 1982 Sep 25;257(18):10869-73 PMID: 7050123
  8. Regulation of protein accumulation in cultured cells.
    Biochem J. 1982 Nov 15;208(2):275-87 PMID: 6760858
  9. Tertiary structures, receptor binding, and antigenicity of insulinlike growth factors.
    Fed Proc. 1983 Jun;42(9):2592-7 PMID: 6189745
  10. High-sensitivity sequencing with a gas-phase sequenator.
    Methods Enzymol. 1983;91:399-413 PMID: 6855591
  11. Mitogen-potentiating action and binding characteristics of insulin and insulin-like growth factors in Chinese hamster fibroblasts.
    Exp Cell Res. 1983 Sep;147(2):369-78 PMID: 6352292
  12. Human chromosomal mapping of genes for insulin-like growth factors I and II and epidermal growth factor.
    Nature. 1984 Aug 30-Sep 5;310(5980):781-4 PMID: 6382023
  13. Comparison of in vivo effects of insulin-like growth factors I and II and of growth hormone in hypophysectomized rats.
    Acta Endocrinol (Copenh). 1985 Feb;108(2):167-74 PMID: 3881885
  14. Production of insulin-like growth factor I and its binding protein by adult rat hepatocytes in primary culture.
    Endocrinology. 1985 Mar;116(3):1094-101 PMID: 2982572
  15. Actions of insulin-like growth factors.
    Annu Rev Physiol. 1985;47:443-67 PMID: 2986538
  16. Insulin-like growth factors I and II in fetal and adult bovine serum. Purification, primary structures, and immunological cross-reactivities.
    J Biol Chem. 1986 Jan 15;261(2):569-75 PMID: 3941093
  17. Mitogenic activity and receptor reactivity of hybrid molecules containing portions of the insulin-like growth factor I (IGF-I), IGF-II, and insulin molecules.
    Diabetes. 1986 Mar;35(3):355-61 PMID: 3005095
  18. Isolation and partial characterization of six somatomedin-like peptides from human plasma Cohn fraction IV.
    Acta Endocrinol (Copenh). 1986 Feb;111(2):271-84 PMID: 3953237
  19. Purification and partial sequence analysis of insulin-like growth factor-1 from bovine colostrum.
    Biochem J. 1986 Jan 1;233(1):207-13 PMID: 3954725
  20. Comparative binding of bovine, human and rat insulin-like growth factors to membrane receptors and to antibodies against human insulin-like growth factor-1.
    Biochem J. 1986 Jan 1;233(1):215-21 PMID: 3513757
  21. Binding properties and biological potencies of insulin-like growth factors in L6 myoblasts.
    Biochem J. 1986 Jan 1;233(1):223-30 PMID: 3954726
  22. Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides.
    J Biol Chem. 1986 Apr 15;261(11):4828-32 PMID: 2937782
  23. Isolation and characterization of variant IGF-1 as well as IGF-2 from adult human brain.
    FEBS Lett. 1986 May 26;201(1):46-50 PMID: 3709807
  24. Characterization of somatomedins from human fetal brain: identification of a variant form of insulin-like growth factor I.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4904-7 PMID: 3460078
  25. Insulin-like growth factor I stimulates growth in normal growing rats.
    Eur J Pharmacol. 1986 Jun 5;125(1):143-6 PMID: 3732389
  26. Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors.
    Nucleic Acids Res. 1986 Oct 24;14(20):7873-82 PMID: 3774549
  27. Identification of an insulin-like growth factor-binding protein in human cerebrospinal fluid with a selective affinity for IGF-II.
    FEBS Lett. 1986 Nov 24;208(2):439-44 PMID: 3536579
  28. Circulating forms of human foetal somatomedin.
    Acta Endocrinol (Copenh). 1987 Jan;114(1):37-40 PMID: 3811771
  29. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.
    J Biol Chem. 1978 Apr 25;253(8):2769-76 PMID: 632300
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2510095
Published
1988-04-01
Pages
95-103
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148968
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2510095 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2