Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 7873-82

Bell GI, Stempien MM, Fong NM, Rall LB

Abstract

Complementary DNAs encoding mouse liver insulin-like growth factor I (IGF-I) have been isolated and sequenced. Alternative RNA splicing results in the synthesis of two types of mouse IGF-I precursor that differ in the size and sequence of the COOH-terminal peptide. The sequences of the signal peptides, IGF-I moieties and the first 16 amino acids of the COOH-terminal peptides or E-domains of the two precursors are identical. The sequence difference results from the presence in preproIGF-IB mRNA of a 52 base insertion which introduces a 17 amino acid segment into the COOH-terminal peptide of preproIGF-IB and also causes a shift in the reading frame of the mRNA. As a consequence of this insertion, the COOH-terminal 19 and 25 amino acids of mouse preproIGF-IA and -IB, respectively, are different. The sequences of mouse and human preproIGF-IA are highly conserved and possess 94% identity. In contrast, the sequences of mouse and human preproIGF-IB are quite different in the region of the COOH-terminal peptide. A comparison of the sequences of mouse and human preproIGF-IB mRNA indicates that they are generated by different molecular mechanisms.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Insulin-Like Growth Factor I/genetics Liver/physiology Mice Protein Precursors/genetics RNA, Messenger/genetics Sequence Homology, Nucleic Acid Somatomedins/genetics

Chemicals

Protein Precursors RNA, Messenger Somatomedins Insulin-Like Growth Factor I DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bell G I

Stempien M M

Fong N M

Rall L B

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26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.20.7873

Published

1986-10-24

Pages

7873-82

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311821

Subset

Databases

GENBANK

X04480, X04482

External Links

PubMed source DOI: 10.1093/nar/14.20.7873 PMC Full Text View journal details

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Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors.

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