Location and nucleotide sequence of two tRNA genes and a tRNA pseudo-gene in the maize mitochondrial genome: evidence for the transcription of a chloroplast gene in mitochondria.

Current genetics ·Vol. 13 ·No. 3 ·1988-03-00 ·Pages 247-54

Wintz H, Grienenberger JM, Weil JH, Lonsdale DM

Abstract

We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of this tRNA(Cys)(GCA) gene with its chloroplast counterpart in wheat suggests that it may be part of a chloroplast DNA insertion into the mitochondrial genome. This gene has been found to be transcribed in the mitochondrion. Two tRNA genes are located on the 5.7 kb BamHI fragment, separated from each other by 250 bp. One is a mitochondrial tRNA(Ser)(GCU) gene. The other, a non-transcribed tRNA(Phe)-like gene, is interrupted by a 49 base-pair inserted DNA sequence in the variable loop and has a Leu (UAA) anticodon.

MeSH Terms

Base Sequence Chloroplasts/metabolism Cloning, Molecular Cosmids DNA, Mitochondrial/genetics Genes Mitochondria/metabolism Molecular Sequence Data Nucleic Acid Conformation Plants/genetics Pseudogenes RNA, Transfer/genetics Transcription, Genetic Zea mays/genetics

Chemicals

DNA, Mitochondrial RNA, Transfer

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wintz H

Institut de Biologie Moléculaire des Plantes, Strasbourg, France.

Grienenberger J M

Weil J H

Lonsdale D M

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31 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00387771

Published

1988-03-00

Pages

247-54

Language

English

Region

United States

NLM ID

8004904

Subset

Databases

GENBANK

X07379

External Links

PubMed source DOI: 10.1007/BF00387771 View journal details

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Location and nucleotide sequence of two tRNA genes and a tRNA pseudo-gene in the maize mitochondrial genome: evidence for the transcription of a chloroplast gene in mitochondria.

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