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PMID: 3355508 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Two genes encoding 'minor' legumin polypeptides in pea (Pisum sativum L.). Characterization and complete sequence of the LegJ gene.

The Biochemical journal ·Vol. 250 ·No. 1 ·1988-02-15 ·Pages 15-24

Gatehouse JA, Bown D, Gilroy J, Levasseur M, Castleton J, Ellis TH

Abstract

A genomic clone from pea (Pisum sativum L.) contains all of one gene encoding a 'minor' (B-type) legumin polypeptide, and most of a second very similar gene. The two genes, designated LegJ and LegK, are arranged in tandem, separated by approx. 6 kb. A complete sequence of gene LegJ and its flanking sequences is given, with as much of the sequence of gene LegK as is present on the genomic clone. Hybridization of 3' flanking sequence probes to seed mRNA, and sequence comparisons with cDNA species, suggested that gene LegJ, and probably gene LegK, was expressed. The partial amino acid sequences of 'minor' legumin alpha- and beta-polypeptides were used to confirm the identity of these genes. The transciption start in gene LegJ was mapped. The 5' flanking sequence of gene LegJ contains a sequence conserved in legumin genes from pea and other species, which is likely to have functional significance in control of gene expression. Sequence comparisons with legumin genes and cDNA species from Vicia faba and soya bean show that separation of legumin genes into A- and B-type subfamilies occurred before separation of the Viciae and Glycinae tribes.

MeSH Terms
Base Sequence DNA/genetics DNA, Circular/genetics Fabaceae/genetics Gene Expression Regulation Genes Molecular Sequence Data Nucleic Acid Hybridization Peptides/genetics Plant Proteins Plant Proteins, Dietary/genetics Plants, Medicinal Species Specificity Transcription, Genetic
Chemicals
DNA, Circular Peptides Plant Proteins Plant Proteins, Dietary legumin protein, plant DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gatehouse J A
Department of Botany, Durham University, U.K.
Bown D
Gilroy J
Levasseur M
Castleton J
Ellis T H
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2500015
Published
1988-02-15
Pages
15-24
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148808
Subset
IM
Databases
GENBANK
X07014, X07015, Y00715
External Links
PubMed source DOI: 10.1042/bj2500015 PMC Full Text View journal details
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