Home LiteratureArticle Details
PMID: 3306681 Published · ppublish English Journal Article

Intramolecular recombination between partially homologous sequences in Escherichia coli and Xenopus laevis oocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 18 ·1987-09-00 ·Pages 6496-500

Abastado JP, Darche S, Godeau F, Cami B, Kourilsky P

Abstract

We describe a system to analyze the individual contribution of a single physical DNA end on intramolecular recombination between partially homologous sequences. We took advantage of this partial sequence divergence to measure the distance separating the DNA end from the final recombination event. We show that a single physical DNA end stimulates recombination when located in a region of homology. Recombination frequency decreases gradually with the distance from the DNA end. A recombinational hot spot is found at the end of the region of homology. A large insertion of unrelated DNA interferes asymmetrically with this process, suggesting that a recombinogenic signal propagates along the region of homology.

MeSH Terms
Animals Chromosome Mapping DNA, Bacterial/genetics Escherichia coli/genetics Oocytes/physiology Plasmids Recombination, Genetic Sequence Homology, Nucleic Acid Xenopus laevis/genetics
Chemicals
DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Abastado J P
Darche S
Godeau F
Cami B
Kourilsky P
References (36)
36 references, click to expand
  1. Genetic exchanges between partially homologous nucleotide sequences: possible implications for multigene families.
    Biochimie. 1983 Feb;65(2):85-93 PMID: 6405807
  2. Plasmidic recombination in Escherichia coli K-12: the role of recF gene function.
    Mol Gen Genet. 1983;189(3):471-4 PMID: 6346018
  3. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  4. Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli.
    Nucleic Acids Res. 1983 Aug 25;11(16):5661-9 PMID: 6310510
  5. Recombination between sequences in nonhomologous positions.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5675-9 PMID: 6351064
  6. Insertions, deletions and mismatches in heteroduplex DNA made by recA protein.
    Cell. 1983 Dec;35(2 Pt 1):511-20 PMID: 6317195
  7. Activation of a Qa/Tla class I major histocompatibility antigen gene is a general feature of oncogenesis in the mouse.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):756-60 PMID: 6318117
  8. Use of a cloned DNA fragment to analyze the fate of donor DNA in transformation of Streptococcus pneumoniae.
    J Bacteriol. 1984 Jun;158(3):1175-8 PMID: 6327632
  9. Recombination-dependent recircularization of linearized pBR322 plasmid DNA following transformation of Escherichia coli.
    Mol Gen Genet. 1984;194(1-2):211-8 PMID: 6374376
  10. Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
    Mol Cell Biol. 1984 Jun;4(6):1020-34 PMID: 6330525
  11. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  12. Lack of association between intrachromosomal gene conversion and reciprocal exchange.
    Nature. 1984 Aug 30-Sep 5;310(5980):748-53 PMID: 6088989
  13. Processing of complex heteroduplexes in Escherichia coli and Cos-1 monkey cells.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5792-6 PMID: 6091108
  14. Evidence for inclusion of regions of nonhomology in heteroduplex products of bacteriophage lambda recombination.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7180-4 PMID: 6239288
  15. DNA sequence of the mouse H-2Dd transplantation antigen gene.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1175-9 PMID: 3856254
  16. Mouse histocompatibility genes: structure and organisation of a Kd gene.
    EMBO J. 1983;2(2):245-54 PMID: 11894934
  17. The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.
    EMBO J. 1983;2(3):453-62 PMID: 11894963
  18. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  19. Some features of genetic recombination in procaryotes.
    Annu Rev Genet. 1978;12:47-68 PMID: 371531
  20. The use of gene fusions to study the expression of malT the positive regulator gene of the maltose regulon.
    J Mol Biol. 1979 Aug 15;132(3):521-34 PMID: 118263
  21. Initiation of genetic recombination: homologous pairing between duplex DNA molecules promoted by recA protein.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3962-6 PMID: 6449004
  22. Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region.
    Gene. 1980 Dec;12(3-4):235-41 PMID: 6265317
  23. Yeast transformation: a model system for the study of recombination.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8 PMID: 6273866
  24. Polar branch migration promoted by recA protein: effect of mismatched base pairs.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):762-6 PMID: 6950427
  25. A molecular map of the immune response region from the major histocompatibility complex of the mouse.
    Nature. 1982 Nov 4;300(5887):35-42 PMID: 6290895
  26. A cDNA clone containing the entire coding sequence of a mouse H-2Kd histocompatibility antigen.
    Nucleic Acids Res. 1983 Mar 11;11(5):1567-77 PMID: 6298749
  27. Comparison of the cloned H-2Kbm1 variant gene with the H-2Kb gene shows a cluster of seven nucleotide differences.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):2007-11 PMID: 6300887
  28. Origins of gene conversion and reciprocal exchange in Ascobolus.
    Cold Spring Harb Symp Quant Biol. 1984;49:13-21 PMID: 6597751
  29. Genes of the RecE and RecF pathways of conjugational recombination in Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1984;49:453-62 PMID: 6397300
  30. Rec-dependent and Rec-independent recombination of plasmid-borne duplications in Escherichia coli K12.
    Mol Gen Genet. 1985;199(3):518-23 PMID: 2993800
  31. Intramolecular recombination of linear DNA catalyzed by the Escherichia coli RecE recombination system.
    J Mol Biol. 1985 Dec 5;186(3):515-25 PMID: 3005590
  32. Double-strand gap repair results in homologous recombination in mouse L cells.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1762-6 PMID: 3006069
  33. Plasmid recombination intermediates generated in a Saccharomyces cerevisiae cell-free recombination system.
    Mol Cell Biol. 1985 Sep;5(9):2361-8 PMID: 3915541
  34. Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over.
    EMBO J. 1986 Sep;5(9):2123-9 PMID: 3023057
  35. Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.
    Mol Cell Biol. 1986 Jun;6(6):2053-61 PMID: 2946937
  36. Roles of double-strand breaks in generalized genetic recombination.
    Prog Nucleic Acid Res Mol Biol. 1986;33:169-94 PMID: 2948217
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.18.6496
Published
1987-09-00
Pages
6496-500
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299104
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.18.6496 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2