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PMID: 3294796 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the sppA gene coding for protease IV, a signal peptide peptidase of Escherichia coli.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2523-8

Suzuki T, Itoh A, Ichihara S, Mizushima S

Abstract

The sppA gene codes for protease IV, a signal peptide peptidase of Escherichia coli. Using the gene cloned on a plasmid, we constructed an E. coli strain carrying the ampicillin resistance gene near the chromosomal sppA gene and an sppA deletion strain in which the deleted portion was replaced by the kanamycin resistance gene. Using these strains, we mapped the sppA gene at 38.5 min on the chromosome, the gene order being katE-xthA-sppA-pncA. Although digestion of the signal peptide that accumulated in the cell envelope fraction was considerably slower in the deletion mutant than in the sppA+ strain, it was still significant, suggesting the participation of another envelope protease(s) in signal peptide digestion.

MeSH Terms

Chromosome Deletion Chromosome Mapping Cloning, Molecular Endopeptidases/genetics Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genetic Engineering Membrane Proteins Peptide Hydrolases/genetics Serine Endopeptidases beta-Lactamases/genetics

Chemicals

Membrane Proteins Endopeptidases Peptide Hydrolases Serine Endopeptidases type I signal peptidase protease IV beta-Lactamases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Suzuki T

Itoh A

Ichihara S

Mizushima S

References (35)

35 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.6.2523-2528.1987

Published

1987-06-00

Pages

2523-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC212110

Subset

External Links

PubMed source DOI: 10.1128/jb.169.6.2523-2528.1987 PMC Full Text View journal details

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Characterization of the sppA gene coding for protease IV, a signal peptide peptidase of Escherichia coli.

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