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PMID: 3290202 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

Journal of bacteriology ·Vol. 170 ·No. 7 ·1988-07-00 ·Pages 3294-6

Shevell DE, Abou-Zamzam AM, Demple B, Walker GC

Abstract

We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.

MeSH Terms
Alkylation Bacterial Proteins/genetics Chromosome Deletion DNA Repair DNA, Bacterial/metabolism Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes, Bacterial Methylation Methyltransferases/metabolism Mutation O(6)-Methylguanine-DNA Methyltransferase Transcription Factors
Chemicals
Bacterial Proteins DNA, Bacterial Escherichia coli Proteins Transcription Factors Methyltransferases Ada protein, E coli O(6)-Methylguanine-DNA Methyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shevell D E
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Abou-Zamzam A M
Demple B
Walker G C
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.7.3294-3296.1988
Published
1988-07-00
Pages
3294-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211286
Subset
IM
Grants
NIEHS NIH HHS · T32ES07020 · United States
External Links
PubMed source DOI: 10.1128/jb.170.7.3294-3296.1988 PMC Full Text View journal details
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