Evaluation of four methods for enumeration of Vibrio parahaemolyticus.

Applied and environmental microbiology ·Vol. 54 ·No. 2 ·1988-02-00 ·Pages 617-8

DePaola A, Hopkins LH, McPhearson RM

Abstract

Two membrane filter (MF) and two most-probable-number methods for enumerating Vibrio parahaemolyticus were compared. The MF methods used elevated-temperature incubations (41 and 42 degrees C) and were more specific than the most-probable-number methods (conducted at 35 degrees C). The MF method with a hydrophobic grid and a repair step was most effective.

MeSH Terms

Animals Bacteriological Techniques Predictive Value of Tests Seawater Shellfish Temperature Vibrio parahaemolyticus/growth & development Water Microbiology

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

DePaola A

Fishery Research Branch, Food and Drug Administration, Dauphin Island, Alabama 36528.

Hopkins L H

McPhearson R M

References (7)

7 references, click to expand

A survey on the occurrence of Vibrio parahaemolyticus on fish and shellfish, marketed in the Netherlands.
J Hyg (Lond). 1970 Jun;68(2):189-96 PMID: 5270202
Occurrence of Vibrio parahaemolyticus and related hemolytic vibrios in marine environments of Washington State.
Appl Microbiol. 1970 Aug;20(2):179-86 PMID: 4921057
Occurrence of Vibrio parahaemolyticus in estuarine waters and oysters of New Hampshire.
Appl Microbiol. 1971 May;21(5):965-6 PMID: 5574329
Elevated temperature method for recovery of Vibrio cholerae from oysters (Crassostrea gigas).
Appl Environ Microbiol. 1987 May;53(5):1181-2 PMID: 3606095
Method for the detection of injured Vibrio parahaemolyticus in seafoods.
Appl Environ Microbiol. 1978 Jun;35(6):1121-7 PMID: 354527
Occurrence of Vibrio parahaemolyticus in selected marine invertebrates, sediment, and seawater around Alexandria, Egypt.
Can J Microbiol. 1982 Nov;28(11):1261-4 PMID: 7151020
Membrane filter procedure for enumeration of Vibrio parahaemolyticus.
Appl Environ Microbiol. 1976 Nov;32(5):679-84 PMID: 984837

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.54.2.617-618.1988

Published

1988-02-00

Pages

617-8

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC202508

Subset

External Links

PubMed source DOI: 10.1128/aem.54.2.617-618.1988 PMC Full Text View journal details

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Evaluation of four methods for enumeration of Vibrio parahaemolyticus.

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