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PMID: 3275869 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Distinct binding sites for zinc and double-stranded RNA in the reovirus outer capsid protein sigma 3.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 273-83

Schiff LA, Nibert ML, Co MS, Brown EG, Fields BN

Abstract

By atomic absorption analysis, we determined that the reovirus outer capsid protein sigma 3, which binds double-stranded RNA (dsRNA), is a zinc metalloprotein. Using Northwestern blots and a novel zinc blotting technique, we localized the zinc- and dsRNA-binding activities of sigma 3 to distinct V8 protease-generated fragments. Zinc-binding activity was contained within an amino-terminal fragment that contained a transcription factor IIIA-like zinc-binding sequence, and dsRNA-binding activity was associated with a carboxy-terminal fragment. By these techniques, new zinc- and dsRNA-binding activities were also detected in reovirus core proteins. A sequence similarity was observed between the catalytic site of the picornavirus proteases and the transcription factor IIIA-like zinc-binding site within sigma 3. We suggest that the zinc- and dsRNA-binding activities of sigma 3 may be important for its proposed regulatory effects on viral and host cell transcription and translation.

MeSH Terms
Amino Acid Sequence Binding Sites Capsid/metabolism Capsid Proteins Electrophoresis, Polyacrylamide Gel/methods Molecular Sequence Data Peptide Fragments/metabolism Peptide Hydrolases/metabolism RNA, Double-Stranded/metabolism RNA-Binding Proteins Reoviridae/metabolism,ultrastructure Structure-Activity Relationship Viral Proteins/metabolism Zinc/metabolism
Chemicals
Capsid Proteins Peptide Fragments RNA, Double-Stranded RNA-Binding Proteins Viral Proteins sigma protein 3, Reovirus Peptide Hydrolases Zinc
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schiff L A
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Nibert M L
Co M S
Brown E G
Fields B N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.273-283.1988
Published
1988-01-00
Pages
273-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363116
Subset
IM
Grants
NIGMS NIH HHS · T32 GM007753 · United States
NIGMS NIH HHS · 5 T32 GM07753-09 · United States
NIAID NIH HHS · AI13178 · United States
NIDDK NIH HHS · DK36837 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.1.273-283.1988 PMC Full Text View journal details
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