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PMID: 6492267 Published · ppublish English Journal Article

Reovirus type 3 genome segment S4: nucleotide sequence of the gene encoding a major virion surface protein.

Journal of virology ·Vol. 52 ·No. 3 ·1984-12-00 ·Pages 984-7

Giantini M, Seliger LS, Furuichi Y, Shatkin AJ

Abstract

A full-length cDNA copy of reovirus double-stranded RNA genome segment S4 which codes for a major virion structural polypeptide, sigma 3, has been completely sequenced. The 1,196-nucleotide cDNA contains a single long open reading frame in the plus strand extending 1,095 nucleotides from the 5'-proximal A-T-G to a single stop codon. This corresponds to translation of 92% of the S4 gene. The inferred sigma 3 polypeptide is hydrophilic and consists of 365 amino acids, totalling 41,164 daltons.

MeSH Terms
Amino Acid Sequence Base Sequence Capsid/genetics Genes Genes, Viral Mammalian orthoreovirus 3/genetics Reoviridae/genetics Solubility
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Giantini M
Seliger L S
Furuichi Y
Shatkin A J
References (29)
29 references, click to expand
  1. RNA polymerase activity in purified reoviruses.
    Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462-9 PMID: 4179180
  2. Polypeptide components of virions, top component and cores of reovirus type 3.
    Virology. 1969 Dec;39(4):791-810 PMID: 4311639
  3. Regulation of the reovirus RNA transcriptase by a viral capsomere protein.
    Virology. 1972 Jun;48(3):648-54 PMID: 5064083
  4. Studies on the effect of chymotrypsin on reovirions.
    Virology. 1972 Sep;49(3):700-15 PMID: 4672416
  5. Transcription by infectious subviral particles of reovirus.
    J Virol. 1972 Oct;10(4):698-706 PMID: 4673489
  6. Studies on the amino and carboxyl terminal amino acid sequences of reovirus capsid polypeptides.
    Virology. 1973 Mar;52(1):174-86 PMID: 4803393
  7. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6 PMID: 1054511
  8. Reovirus-coded polypeptides in infected cells: isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively.
    Virology. 1976 Apr;70(2):411-24 PMID: 1266045
  9. Evidence for a glycoprotein in reovirus.
    Virology. 1976 Jul 15;72(2):308-21 PMID: 948864
  10. Sequences and properties of two ribosome binding sites from the small size class of reovirus messenger RNA.
    J Biol Chem. 1977 Oct 10;252(19):6895-908 PMID: 893449
  11. Detection of 5-methylcytosine in DNA sequences.
    Nucleic Acids Res. 1978 May;5(5):1479-85 PMID: 351561
  12. The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3.
    Virology. 1978 Sep;89(2):578-93 PMID: 716218
  13. Genetics of reovirus: identification of the ds RNA segments encoding the polypeptides of the mu and sigma size classes.
    Virology. 1978 Sep;89(2):594-604 PMID: 716219
  14. Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease.
    J Biol Chem. 1979 Feb 25;254(4):1408-13 PMID: 368070
  15. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  16. 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate.
    Gene. 1980 Jul;10(2):177-83 PMID: 6248422
  17. Assignment of reovirus mRNA ribosome binding sites to virion genome segments by nucleotide sequence analyses.
    Nucleic Acids Res. 1980 Jan 25;8(2):337-50 PMID: 7422544
  18. Terminal structure of reovirus RNAs.
    J Gen Virol. 1981 Aug;55(Pt 2):393-403 PMID: 6270271
  19. Role of the S4 gene in the establishment of persistent reovirus infection in L cells.
    Cell. 1982 Mar;28(3):605-12 PMID: 7074685
  20. Reovirus transcriptase and capping enzymes are active in intact virions.
    Virology. 1982 Apr 15;118(1):157-68 PMID: 7080437
  21. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  22. Sequence at both termini of the 10 genes of reovirus serotype 3 (strain Dearing).
    Virology. 1982 Sep;121(2):307-19 PMID: 6927854
  23. Reovirus inhibition of cellular RNA and protein synthesis: role of the S4 gene.
    Virology. 1982 Oct 30;122(2):381-91 PMID: 6183822
  24. Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7644-8 PMID: 6961439
  25. Molecular cloning of double-stranded RNA virus genomes.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):373-7 PMID: 6300836
  26. Nucleotide sequence of reovirus genome segment S3, encoding non-structural protein sigma NS.
    Nucleic Acids Res. 1983 Sep 24;11(18):6399-408 PMID: 6312421
  27. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  28. Two initiation sites detected in the small s1 species of reovirus mRNA by dipeptide synthesis in vitro.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1084-8 PMID: 6583697
  29. mRNA discrimination in extracts from uninfected and reovirus-infected L-cells.
    J Virol. 1984 Jul;51(1):215-22 PMID: 6328041
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.52.3.984-987.1984
Published
1984-12-00
Pages
984-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254625
Subset
IM
Databases
GENBANK
K02739
External Links
PubMed source DOI: 10.1128/JVI.52.3.984-987.1984 PMC Full Text View journal details
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