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PMID: 327267 Published · ppublish English Journal Article

Altered dihydrofolate reductase in fol regulatroy mutants of Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 151 ·No. 2 ·1977-03-07 ·Pages 215-9

Sheldon R

Abstract

Three spontaneous fol regulatory mutants contain dihydrofolate reductase molecules which differ in physical properties from enzymes of their parent strains. The enzymes were purified over 100-fold by affinity chromatography and were shown to differ in vitro in thermolability and in affinity for trimethoprim, a competitive inhibitor of the enzyme. These results indicate that some fol regulatroy mutations occur in the structural gene for dihydrofolate reductase.

MeSH Terms
Chromatography, Affinity Escherichia coli/enzymology Genes Genes, Regulator Molecular Conformation Mutation Tetrahydrofolate Dehydrogenase/biosynthesis Trimethoprim/metabolism
Chemicals
Trimethoprim Tetrahydrofolate Dehydrogenase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Sheldon R
References (12)
12 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00338697
Published
1977-03-07
Pages
215-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00338697
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