Home LiteratureArticle Details
PMID: 3264069 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Erythropoietin changes the globin program of an interleukin 3-dependent multipotential cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 23 ·1988-12-00 ·Pages 9091-5

Enver T, Nakamoto B, Karlinsey J, Josephson B, Greenberger J, Papayannopoulou T

Abstract

B6SUtA is a factor-dependent murine cell line of adult origin displaying the functional properties of a multipotent hematopoietic stem cell. We analyzed the globin programs of B6SUtA cells undergoing erythroid differentiation in both suspension and clonal cultures. In the absence of added erythropoietin, a small number of hemoglobinized cells were present, and these expressed predominantly embryonic globin. Addition of erythropoietin increased the number and maturation of hemoglobinized cells and led to a preferential augmentation of adult globin. Analysis of individual B6SUtA erythroid bursts showed that embryonic and adult globin can be expressed in cells derived from a single progenitor. Furthermore, by studying globin expression in cultured cells from mouse embryos, we found that the globin programs of B6SUtA cells are similar to those of erythroid progenitors at the period of transition from yolk sac to fetal liver erythropoiesis. Since B6SUtA cells are derived from adult bone marrow and they have the capacity to express embryonic globin, we speculate that the globin locus is not irreversibly modified during development and that adult cells at early stages of erythroid differentiation can transiently express ontogenetically primitive globin programs.

MeSH Terms
Animals Cell Differentiation/drug effects Cell Line Erythropoietin/pharmacology Gene Expression Regulation Genes Globins/genetics Humans Interleukin-3/pharmacology Mice RNA, Messenger/genetics,isolation & purification Recombinant Proteins/pharmacology
Chemicals
Interleukin-3 RNA, Messenger Recombinant Proteins Erythropoietin Globins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Enver T
Division of Medical Genetics, University of Washington, Seattle 98195.
Nakamoto B
Karlinsey J
Josephson B
Greenberger J
Papayannopoulou T
References (32)
32 references, click to expand
  1. Activation of hemoglobin C synthesis in sheep marrow culture.
    Science. 1973 Apr 20;180(4083):310-2 PMID: 4735594
  2. Growth of murine multipotent stem cells in a simple "serum-free" culture system: role of interleukin-3, erythropoietin, and hemin.
    Exp Hematol. 1988 May;16(4):250-5 PMID: 3258824
  3. Hemoglobin ontogeny during normal mouse fetal development.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2853-7 PMID: 111244
  4. Cellular regulation of hemoglobin switching: evidence for inverse relationship between fetal hemoglobin synthesis and degree of maturity of human erythroid cells.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6420-4 PMID: 293729
  5. Proportion of fetal hemoglobin synthesis decreases during erythroid cell maturation.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2757-61 PMID: 6156456
  6. Human globin chain separation by isoelectric focusing.
    J Biochem Biophys Methods. 1979;1(1):45-57 PMID: 551975
  7. Is erythropoietin the only factor which regulates late erythroid differentiation?
    Nature. 1981 Jan 15;289(5794):184-6 PMID: 7453819
  8. Globin-chain affinity chromatography on Sepharose-haptoglobin: a new method of study of hemoglobin synthesis in reticulocytes, in bone marrow and in colonies of erythroid precursors.
    Eur J Biochem. 1980 Dec;112(3):513-9 PMID: 7460935
  9. Synthesis of hemoglobin F in adult simian erythroid progenitor-derived colonies.
    J Clin Invest. 1982 Oct;70(4):752-61 PMID: 6181096
  10. Renewal and commitment to differentiation of hemopoietic stem cells (an interpretive review).
    Blood. 1983 May;61(5):823-9 PMID: 6338973
  11. Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.
    Proc Natl Acad Sci U S A. 1983 May;80(10):2931-5 PMID: 6574462
  12. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
    Cell. 1983 Oct;34(3):865-79 PMID: 6313211
  13. Bipotential murine hemopoietic cell line (NFS-60) that is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin.
    Exp Hematol. 1988 May;16(4):256-61 PMID: 2452092
  14. A STOCHASTIC MODEL OF STEM CELL PROLIFERATION, BASED ON THE GROWTH OF SPLEEN COLONY-FORMING CELLS.
    Proc Natl Acad Sci U S A. 1964 Jan;51:29-36 PMID: 14104600
  15. A method for isolation of intact, translationally active ribonucleic acid.
    DNA. 1983;2(4):329-35 PMID: 6198133
  16. Developmental regulation of a cloned adult beta-globin gene in transgenic mice.
    Nature. 1985 May 23-29;315(6017):338-40 PMID: 3858676
  17. Interleukin 3 promotes erythroid burst formation in "serum-free" cultures without detectable erythropoietin.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3291-5 PMID: 3923475
  18. Studies of Hb F in adult nonanemic baboons: Hb F expression in erythroid colonies decreases as the level of maturation of erythroid progenitors advances.
    Exp Hematol. 1985 Oct;13(9):919-25 PMID: 2412874
  19. A self-renewing, bipotential erythroid/mast cell progenitor in continuous cultures of normal murine bone marrow.
    J Cell Physiol. 1985 Oct;125(1):10-8 PMID: 2931443
  20. Activity in fetal bovine serum that stimulates erythroid colony formation in fetal mouse livers is insulinlike growth factor I.
    J Clin Invest. 1985 Oct;76(4):1643-8 PMID: 4056043
  21. Lineage promiscuity in hemopoietic differentiation and leukemia.
    Blood. 1986 Jan;67(1):1-11 PMID: 3079640
  22. Hemoglobin switching during murine embryonic development: evidence for two populations of embryonic erythropoietic progenitor cells.
    Blood. 1986 Mar;67(3):716-21 PMID: 3947744
  23. An embryonic pattern of expression of a human fetal globin gene in transgenic mice.
    Nature. 1986 Feb 20-26;319(6055):685-9 PMID: 3951540
  24. Purified interleukin-3 and erythropoietin support the terminal differentiation of hemopoietic progenitors in serum-free culture.
    Blood. 1986 Apr;67(4):1002-6 PMID: 3082386
  25. Properties of the earliest clonogenic hemopoietic precursors to appear in the developing murine yolk sac.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3851-4 PMID: 3012535
  26. Rapid reprogramming of globin gene expression in transient heterokaryons.
    Cell. 1986 Aug 15;46(4):591-602 PMID: 3731277
  27. Self-renewal and differentiation of interleukin-3-dependent multipotent stem cells are modulated by stromal cells and serum factors.
    Differentiation. 1986;31(2):111-8 PMID: 3091439
  28. Expression of the two adult beta-globin genes in mouse yolk sac and fetal liver erythrocytes.
    Dev Biol. 1987 Jan;119(1):299-301 PMID: 2431938
  29. Cytokine-dependent granulocytic differentiation. Regulation of proliferative and differentiative responses in a murine progenitor cell line.
    J Immunol. 1987 Jun 1;138(11):3829-35 PMID: 2438328
  30. Stimulation of fetal hemoglobin synthesis by erythropoietin in baboons.
    N Engl J Med. 1987 Aug 13;317(7):415-20 PMID: 2441258
  31. The globin gene expression program in the hamster embryo.
    Exp Hematol. 1988 Jan;16(1):1-4 PMID: 3335231
  32. Differentiation in erythroleukemic cells and their somatic hybrids.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):98-102 PMID: 164031
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.23.9091
Published
1988-12-00
Pages
9091-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282669
Subset
IM
Grants
NIDDK NIH HHS · DK30852 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.23.9091 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2