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PMID: 6574462 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 10 ·1983-05-00 ·Pages 2931-5

Greenberger JS, Sakakeeny MA, Humphries RK, Eaves CJ, Eckner RJ

Abstract

Multipotential hematopoietic progenitor cell lines have been established from nonadherent cell populations removed from continuous mouse bone marrow cultures. Clonal sublines of lines B6SUtA or B6JUt derived from single cells formed mixed colonies containing erythroid cells, neutrophil-granulocytes, and basophil/mast cells in semisolid medium containing erythropoietin and conditioned medium from pokeweed mitogen-stimulated spleen cells. Each of several subclones of cell line Ro cl formed colonies containing eosinophils, neutrophil-granulocytes, and basophil/mast cells in semisolid medium. Multipotentiality was maintained in vitro for over 2 1/2 years. In contrast, cell line 32D formed basophil/mast cell colonies with no detectable differentiation to other pathways. Multipotential cell lines did not produce detectable spleen colonies (CFUs) in vivo, nor did intravenous inoculation of up to 5 X 10(7) cells protect lethally irradiated mice from bone marrow failure. Newborn and adult mice inoculated with 5 X 10(7) cells showed no detectable leukemia or solid tumors after one year. Both multipotential and committed basophil/mast cell lines demonstrated absolute dependence upon a source of a growth factor(s) found in medium conditioned by WEHI-3 cells. These cell lines should be of value in studies of the regulation of hematopoietic stem cell differentiation in vitro.

MeSH Terms
Animals Basophils/physiology Cell Line Erythrocytes/physiology Erythropoiesis Granulocytes/physiology Hematopoietic Stem Cells/cytology Mast Cells/physiology Mice Neutrophils/physiology
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Greenberger J S
Sakakeeny M A
Humphries R K
Eaves C J
Eckner R J
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.10.2931
Published
1983-05-00
Pages
2931-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393947
Subset
IM
Grants
NCI NIH HHS · CA25412 · United States
NCI NIH HHS · CA26785 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.10.2931 PMC Full Text View journal details
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