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PMID: 3263621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Unusual branch point selection involved in splicing of the alternatively processed Calcitonin/CGRP-I pre-mRNA.

Nucleic acids research ·Vol. 16 ·No. 20 ·1988-10-25 ·Pages 9513-26

Adema GJ, Bovenberg RA, Jansz HS, Baas PD

Abstract

To study splice site selection in alternative RNA processing we used the human Calcitonin/CGRP-I (CALC-I) gene. Expression of the CALC-I gene in thyroid C-cells results predominantly in calcitonin (CT) mRNA (containing exons 1 to 4) whereas CGRP-I mRNA (containing exons 1,2,3,5 and 6) is the exclusive product in particular nerve cells. We previously reported that a model precursor RNA containing the exon 3 to exon 5 region is predominantly processed into CGRP-I mRNA in vitro using nuclear extracts of three different cell types. To study CT specific processing in Hela cell nuclear extracts we have used precursor RNAs corresponding to the exon 3 to exon 4 region containing only CT specific processing signals. The results revealed the usage of a uridine residue 23 nucleotides upstream of the 3' splice site as the major site of lariat formation in CT specific splicing. The implications of this finding for the alternative, tissue specific processing of the CALC-I pre-mRNA and for branch point selection in general are discussed.

MeSH Terms
Calcitonin/genetics Calcitonin Gene-Related Peptide Exons HeLa Cells Humans Introns Neuropeptides/genetics RNA Caps/metabolism RNA Precursors/metabolism RNA Splicing RNA, Messenger/metabolism
Chemicals
Neuropeptides RNA Caps RNA Precursors RNA, Messenger Calcitonin Calcitonin Gene-Related Peptide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Adema G J
Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, The Netherlands.
Bovenberg R A
Jansz H S
Baas P D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.20.9513
Published
1988-10-25
Pages
9513-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338760
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.20.9513 PMC Full Text View journal details
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