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PMID: 3257824 Published · ppublish English Journal Article

Ligand activation of overexpressed epidermal growth factor receptors transforms NIH 3T3 mouse fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 5 ·1988-03-00 ·Pages 1477-81

Riedel H, Massoglia S, Schlessinger J, Ullrich A

Abstract

The cell surface receptor for the mitogenic peptide epidermal growth factor (EGF) is involved in control of normal cell growth and may play a role in the genesis of human neoplasia such as squamous carcinoma and glioblastoma. Soft-agar growth and focus-formation experiments with NIH 3T3 mouse fibroblasts transfected with an expression plasmid demonstrated the ligand-dependent transforming potential of the human EGF receptor without structural alterations. Activation of overexpressed normal receptor alone appears to be sufficient for transformation of NIH 3T3 cells in vitro.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic/physiopathology Epidermal Growth Factor/physiology ErbB Receptors/physiology Gene Amplification Gene Expression Regulation Ligands Mice Molecular Weight Peptides/physiology Transforming Growth Factors
Chemicals
Ligands Peptides Epidermal Growth Factor Transforming Growth Factors ErbB Receptors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Riedel H
Department of Developmental Biology, Genentech, South San Francisco, CA 94080.
Massoglia S
Schlessinger J
Ullrich A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.5.1477
Published
1988-03-00
Pages
1477-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279794
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.5.1477 PMC Full Text View journal details
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