Home LiteratureArticle Details
PMID: 3906655 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 8014-8

Ebina Y, Edery M, Ellis L, Standring D, Beaudoin J, Roth RA, Rutter WJ

Abstract

We have placed human insulin receptor cDNA into a vector under the control of the simian virus 40 (SV40) early promoter and tested its function by transient expression in microinjected Xenopus oocytes and by expression in stably transformed CHO cells. The precursor and the alpha and beta subunits of the receptor were detected by immunoprecipitation from extracts of these cells. The human insulin receptor expressed in CHO cells specifically binds 125I-labeled insulin but not insulin-like growth factor I, displays insulin-stimulated autophosphorylation of the beta subunit, and mediates insulin-stimulated 2-deoxyglucose uptake. We conclude that the human insulin receptor is synthesized, processed normally, and functional in this heterologous cell system.

MeSH Terms
Animals Antibodies, Monoclonal Cricetinae Cricetulus DNA/genetics Gene Expression Regulation Glucose/metabolism Humans Insulin/metabolism Molecular Weight Oocytes Plasmids Receptor, Insulin/genetics,immunology,physiology Xenopus laevis
Chemicals
Antibodies, Monoclonal Insulin DNA Receptor, Insulin Glucose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ebina Y
Edery M
Ellis L
Standring D
Beaudoin J
Roth R A
Rutter W J
References (22)
22 references, click to expand
  1. Properties of the insulin receptor isolated from liver and fat cell membranes.
    J Biol Chem. 1972 Apr 10;247(7):1980-91 PMID: 5016639
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Insulin interactions with its receptors: experimental evidence for negative cooperativity.
    Biochem Biophys Res Commun. 1973 Nov 1;55(1):154-61 PMID: 4361269
  4. Interaction of cross-linking agents with the insulin effector system of isolated fat cells. Covalent linkage of 125I-insulin to a plasma membrane receptor protein of 140,000 daltons.
    J Biol Chem. 1979 May 10;254(9):3375-81 PMID: 429356
  5. The causes of diabetes.
    Sci Am. 1979 Nov;241(5):62-73 PMID: 228390
  6. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  7. Electrophoretic resolution of three major insulin receptor structures with unique subunit stoichiometries.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7137-41 PMID: 6938960
  8. Insulin receptors, receptor antibodies, and the mechanism of insulin action.
    Recent Prog Horm Res. 1981;37:477-538 PMID: 6269165
  9. Surface redistribution of 125I-insulin in cultured human lymphocytes.
    J Cell Biol. 1981 Oct;91(1):17-25 PMID: 7028760
  10. The structure of insulin receptor and its subunits. Evidence for multiple nonreduced forms and a 210,000 possible proreceptor.
    J Biol Chem. 1982 Sep 10;257(17):10392-9 PMID: 7107610
  11. Insulin stimulation of phosphorylation of the beta subunit of the insulin receptor. Formation of both phosphoserine and phosphotyrosine.
    J Biol Chem. 1982 Sep 10;257(17):9891-4 PMID: 6179940
  12. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  13. Numbers of receptor sites from Scatchard graphs: facts and fantasies.
    Science. 1982 Sep 24;217(4566):1247-9 PMID: 6287580
  14. Insulin receptor: evidence that it is a protein kinase.
    Science. 1983 Jan 21;219(4582):299-301 PMID: 6849137
  15. Monoclonal antibodies to the human insulin receptor block insulin binding and inhibit insulin action.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7312-6 PMID: 6185950
  16. Purification of insulin receptor with full binding activity.
    J Biol Chem. 1983 Apr 25;258(8):5045-9 PMID: 6833291
  17. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
    Mol Cell Biol. 1983 Feb;3(2):280-9 PMID: 6300662
  18. Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.
    Nature. 1984 May 31-Jun 6;309(5967):418-25 PMID: 6328312
  19. Insulin receptors on Chinese hamster ovary (CHO) cells: altered insulin binding to glycosylation mutants.
    Biochem Biophys Res Commun. 1984 Nov 30;125(1):70-5 PMID: 6391490
  20. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
    Nature. 1985 Feb 28-Mar 6;313(6005):756-61 PMID: 2983222
  21. The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling.
    Cell. 1985 Apr;40(4):747-58 PMID: 2859121
  22. Spare gonadotrophin receptors in rat testis.
    Nat New Biol. 1973 Aug 15;244(137):219-21 PMID: 4353683
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.8014
Published
1985-12-00
Pages
8014-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391432
Subset
IM
Grants
NIADDK NIH HHS · AM01393 · United States
NIADDK NIH HHS · AM21344 · United States
NIADDK NIH HHS · AM34926 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.23.8014 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2