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PMID: 3244354 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Stepwise activation of the mouse acetylcholine receptor delta- and gamma-subunit genes in clonal cell lines.

Molecular and cellular biology ·Vol. 8 ·No. 12 ·1988-12-00 ·Pages 5257-67

Crowder CM, Merlie JP

Abstract

We used the DNase I-hypersensitive sites around the mouse acetylcholine receptor delta-subunit gene as a guide toward the cloning and sequencing of delta and gamma transcriptional regulatory regions and as a means to assess chromatin structural activation of the delta- and gamma-subunit genes during myogenesis. Genomic cloning of hypersensitive sites downstream of the delta-subunit gene revealed the presence of the gamma-subunit gene approximately 5 kilobases away; the hypersensitive sites mapped to the 5' end of the gamma-subunit gene. Sequence comparison of restriction fragments containing hypersensitive sites in analogous locations at the 5' ends of the delta- and gamma-subunit genes uncovered little overall homology between the two genomic fragments; however, an 11- of 13-base-pair match between the two sequences was found. Homologs to this sequence were also found to be present in the upstream regions of the chicken alpha- and mouse beta-subunit genes. By RNase protection and primer extension analyses, the delta-subunit gene transcription start site was mapped to 56 base pairs upstream of the initiator ATG codon. Clonal cell lines with various potentials to differentiate to the skeletal muscle phenotype were examined for their hypersensitive site pattern within the delta-gamma locus. Only remote hypersensitive sites flanking the locus appear in pluripotential mesodermal cells. A cell line of determined but inducible myoblasts expressed only one more intergenic site, while in permissively differentiating myoblasts hypersensitive sites were already present at the 5' ends of the delta and gamma genes prior to differentiation. Terminal differentiation resulted in an identical pattern of hypersensitive sites in all muscle cell lines examined so far, with an intergenic site near the gamma-subunit gene being the only site specific to the differentiated muscle phenotype.

MeSH Terms
Animals Base Sequence Cell Line Clone Cells Enhancer Elements, Genetic Gene Expression Regulation Genetic Linkage Macromolecular Substances Mice Molecular Sequence Data Receptors, Cholinergic/genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Macromolecular Substances Receptors, Cholinergic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Crowder C M
Washington University School of Medicine, Department of Pharmacology, St. Louis, Missouri 63110.
Merlie J P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.12.5257-5267.1988
Published
1988-12-00
Pages
5257-67
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365628
Subset
IM
Grants
NIGMS NIH HHS · 5T32 GM07200 · United States
NHLBI NIH HHS · 5T32 HL07275 · United States
Databases
GENBANK
M22380, M22381
External Links
PubMed source DOI: 10.1128/mcb.8.12.5257-5267.1988 PMC Full Text View journal details
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