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PMID: 2989833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activity regulates the levels of acetylcholine receptor alpha-subunit mRNA in cultured chicken myotubes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 13 ·1985-07-00 ·Pages 4558-62

Klarsfeld A, Changeux JP

Abstract

In vitro blocking the spontaneous activity of primary cultures of chicken embryo myotubes with tetrodotoxin increases approximately equal to 2-fold their content in surface acetylcholine receptor. To investigate this effect at the level of gene expression, chicken genomic DNA sequences coding for the acetylcholine receptor alpha subunit were isolated and characterized. They were shown to belong to a single-copy, polymorphic gene with at least two alleles in the chicken strain utilized. Probes derived from these genomic clones were used to quantitate levels of alpha-subunit mRNA. In culture, a 2-day exposure to tetrodotoxin increased these mRNA levels up to 13-fold, a value similar to that observed after denervation of chick leg muscle (approximately equal to 17-fold). Actin mRNA levels varied little in any of these experiments. These results support the notion that membrane electrical activity affects acetylcholine receptor expression by regulating the accumulation of the corresponding mRNAs.

MeSH Terms
Actins/genetics Amino Acid Sequence Animals Base Sequence Cells, Cultured Chick Embryo DNA Restriction Enzymes/metabolism Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Humans Muscle Denervation Muscles/analysis Polymorphism, Genetic RNA, Messenger/metabolism Receptors, Cholinergic/genetics Tetrodotoxin/pharmacology
Chemicals
Actins RNA, Messenger Receptors, Cholinergic Tetrodotoxin DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klarsfeld A
Changeux J P
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.13.4558
Published
1985-07-00
Pages
4558-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391142
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.13.4558 PMC Full Text View journal details
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