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PMID: 32282821 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

The transcription and export complex THO/TREX contributes to transcription termination in plants.

PLoS genetics ·Vol. 16 ·No. 4 ·2020-00-00 ·Pages e1008732

Khan GA, Deforges J, Reis RS, Hsieh YF, Montpetit J, Antosz W, Santuari L, Hardtke CS, Grasser KD, Poirier Y

Abstract

Transcription termination has important regulatory functions, impacting mRNA stability, localization and translation potential. Failure to appropriately terminate transcription can also lead to read-through transcription and the synthesis of antisense RNAs which can have profound impact on gene expression. The Transcription-Export (THO/TREX) protein complex plays an important role in coupling transcription with splicing and export of mRNA. However, little is known about the role of the THO/TREX complex in the control of transcription termination. In this work, we show that two proteins of the THO/TREX complex, namely TREX COMPONENT 1 (TEX1 or THO3) and HYPER RECOMBINATION1 (HPR1 or THO1) contribute to the correct transcription termination at several loci in Arabidopsis thaliana. We first demonstrate this by showing defective termination in tex1 and hpr1 mutants at the nopaline synthase (NOS) terminator present in a T-DNA inserted between exon 1 and 3 of the PHO1 locus in the pho1-7 mutant. Read-through transcription beyond the NOS terminator and splicing-out of the T-DNA resulted in the generation of a near full-length PHO1 mRNA (minus exon 2) in the tex1 pho1-7 and hpr1 pho1-7 double mutants, with enhanced production of a truncated PHO1 protein that retained phosphate export activity. Consequently, the strong reduction of shoot growth associated with the severe phosphate deficiency of the pho1-7 mutant was alleviated in the tex1 pho1-7 and hpr1 pho1-7 double mutants. Additionally, we show that RNA termination defects in tex1 and hpr1 mutants leads to 3'UTR extensions in several endogenous genes. These results demonstrate that THO/TREX complex contributes to the regulation of transcription termination.

MeSH Terms
Amino Acid Oxidoreductases/genetics,metabolism Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Gene Expression Regulation, Plant Transcription Termination, Genetic
Chemicals
Arabidopsis Proteins EMU protein, Arabidopsis PHO1 protein, Arabidopsis TEX1 protein, Arabidopsis Amino Acid Oxidoreductases nopaline synthase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Khan Ghazanfar Abbas ORCID
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland. | School of Biosciences, University of Melbourne, Melbourne, Australia.
Deforges Jules
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Reis Rodrigo S ORCID
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Hsieh Yi-Fang
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Montpetit Jonatan ORCID
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Antosz Wojciech
Department of Cell Biology & Plant Biochemistry, Biochemistry Centre, University of Regensburg, Regensburg, Germany.
Santuari Luca ORCID
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Hardtke Christian S
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Grasser Klaus D ORCID
Department of Cell Biology & Plant Biochemistry, Biochemistry Centre, University of Regensburg, Regensburg, Germany.
Poirier Yves ORCID
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1008732
Published
2020-00-00
Epub
2020-00-13
Pages
e1008732
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC7179932
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pgen.1008732 PMC Full Text View journal details
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