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PMID: 28522613 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dehydration stress extends mRNA 3' untranslated regions with noncoding RNA functions in Arabidopsis.

Genome research ·Vol. 27 ·No. 8 ·2017-00-00 ·Pages 1427-1436

Sun HX, Li Y, Niu QW, Chua NH

Abstract

The 3' untranslated regions (3' UTRs) of mRNAs play important roles in the regulation of mRNA localization, translation, and stability. Alternative cleavage and polyadenylation (APA) generates mRNAs with different 3' UTRs, but the involvement of this process in stress response has not yet been clarified. Here, we report that a subset of stress-related genes exhibits 3' UTR extensions of their mRNAs during dehydration stress. These extended 3' UTRs have characteristics of long noncoding RNAs and likely do not interact with miRNAs. Functional studies using T-DNA insertion mutants reveal that they can act as antisense transcripts to repress expression levels of sense genes from the opposite strand or can activate the transcription or lead to read-through transcription of their downstream genes. Further analysis suggests that transcripts with 3' UTR extensions have weaker poly(A) signals than those without 3' UTR extensions. Finally, we show that their biogenesis is partially dependent on a trans-acting factor FPA. Taken together, we report that dehydration stress could induce transcript 3' UTR extensions and elucidate a novel function for these stress-induced 3' UTR extensions as long noncoding RNAs in the regulation of their neighboring genes.

MeSH Terms
3' Untranslated Regions Abscisic Acid/pharmacology Arabidopsis/drug effects,genetics,growth & development Arabidopsis Proteins/genetics DNA, Bacterial Dehydration Gene Expression Regulation, Plant Genome, Plant Plant Growth Regulators/pharmacology Polyadenylation RNA, Messenger/genetics RNA, Plant/genetics Stress, Physiological
Chemicals
3' Untranslated Regions Arabidopsis Proteins DNA, Bacterial Plant Growth Regulators RNA, Messenger RNA, Plant T-DNA Abscisic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sun Hai-Xi
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065, USA.
Li Yan
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065, USA.
Niu Qi-Wen
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065, USA.
Chua Nam-Hai
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10065, USA.
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1549-5469
DOI
10.1101/gr.218669.116
Published
2017-00-00
Epub
2017-00-18
Pages
1427-1436
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC5538558
Subset
IM
External Links
PubMed source DOI: 10.1101/gr.218669.116 PMC Full Text View journal details
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