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PMID: 18682547 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance.

The Plant cell ·Vol. 20 ·No. 8 ·2008-08-00 ·Pages 2238-51

Li WX, Oono Y, Zhu J, He XJ, Wu JM, Iida K, Lu XY, Cui X, Jin H, Zhu JK

Abstract

Nuclear factor Y (NF-Y) is a ubiquitous transcription factor composed of three distinct subunits (NF-YA, NF-YB, and NF-YC). We found that the Arabidopsis thaliana NFYA5 transcript is strongly induced by drought stress in an abscisic acid (ABA)-dependent manner. Promoter:beta-glucuronidase analyses showed that NFYA5 was highly expressed in vascular tissues and guard cells and that part of the induction by drought was transcriptional. NFYA5 contains a target site for miR169, which targets mRNAs for cleavage or translational repression. We found that miR169 was downregulated by drought stress through an ABA-dependent pathway. Analysis of the expression of miR169 precursors showed that miR169a and miR169c were substantially downregulated by drought stress. Coexpression of miR169 and NFYA5 suggested that miR169a was more efficient than miR169c at repressing the NFYA5 mRNA level. nfya5 knockout plants and plants overexpressing miR169a showed enhanced leaf water loss and were more sensitive to drought stress than wild-type plants. By contrast, transgenic Arabidopsis plants overexpressing NFYA5 displayed reduced leaf water loss and were more resistant to drought stress than the wild type. Microarray analysis indicated that NFYA5 is crucial for the expression of a number of drought stress-responsive genes. Thus, NFYA5 is important for drought resistance, and its induction by drought stress occurs at both the transcriptional and posttranscriptional levels.

MeSH Terms
Abscisic Acid/pharmacology Adaptation, Physiological/drug effects,genetics,physiology Arabidopsis/drug effects,genetics,metabolism Arabidopsis Proteins/genetics,metabolism,physiology CCAAT-Binding Factor/genetics,metabolism,physiology Droughts Gene Expression Regulation, Plant/drug effects MicroRNAs/genetics,metabolism Oligonucleotide Array Sequence Analysis RNA, Messenger/genetics,metabolism Transcription, Genetic/drug effects
Chemicals
Arabidopsis Proteins CCAAT-Binding Factor MIRN169 microRNA, Arabidopsis MicroRNAs NFYA5 protein, Arabidopsis RNA, Messenger Abscisic Acid
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Li Wen-Xue
Key Laboratory of Plant and Soil Interactions, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China.
Oono Youko
Zhu Jianhua
He Xin-Jian
Wu Jian-Min
Iida Kei
Lu Xiao-Yan
Cui Xinping
Jin Hailing
Zhu Jian-Kang
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.108.059444
Published
2008-08-00
Epub
2008-00-05
Pages
2238-51
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2553615
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059138 · United States
NIGMS NIH HHS · R01 GM070795 · United States
NIGMS NIH HHS · R01GM059138 · United States
NIGMS NIH HHS · R01GM070795 · United States
Databases
GEO
GSE12029
External Links
PubMed source DOI: 10.1105/tpc.108.059444 PMC Full Text View journal details
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