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PMID: 17071740 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A pathogen-inducible endogenous siRNA in plant immunity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 47 ·2006-11-21 ·Pages 18002-7

Katiyar-Agarwal S, Morgan R, Dahlbeck D, Borsani O, Villegas A, Zhu JK, Staskawicz BJ, Jin H

Abstract

RNA interference, mediated by small interfering RNAs (siRNAs), is a conserved regulatory process that has evolved as an antiviral defense mechanism in plants and animals. It is not known whether host cells also use siRNAs as an antibacterial defense mechanism in eukaryotes. Here, we report the discovery of an endogenous siRNA, nat-siRNAATGB2, that is specifically induced by the bacterial pathogen Pseudomonas syringae carrying effector avrRpt2. We demonstrate that the biogenesis of this siRNA requires DCL1, HYL1, HEN1, RDR6, NRPD1A, and SGS3. Its induction also depends on the cognate host disease resistance gene RPS2 and the NDR1 gene that is required for RPS2-specified resistance. This siRNA contributes to RPS2-mediated race-specific disease resistance by repressing PPRL, a putative negative regulator of the RPS2 resistance pathway.

MeSH Terms
Animals Arabidopsis/genetics,immunology,microbiology Arabidopsis Proteins/genetics,metabolism Bacterial Proteins/genetics,metabolism Cell Cycle Proteins/genetics,metabolism Plants, Genetically Modified Pseudomonas syringae/pathogenicity RNA, Small Interfering/genetics,metabolism RNA-Binding Proteins/genetics,metabolism RNA-Dependent RNA Polymerase/genetics,metabolism Ribonuclease III/genetics,metabolism
Chemicals
Arabidopsis Proteins Bacterial Proteins Cell Cycle Proteins HEN1 protein, Arabidopsis HYL1 protein, Arabidopsis RNA, Small Interfering RNA-Binding Proteins SGS3 protein, Arabidopsis avrRpt2 protein, Pseudomonas syringae RDR6 protein, Arabidopsis RNA-Dependent RNA Polymerase DCL1 protein, Arabidopsis Ribonuclease III
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Katiyar-Agarwal Surekha
Department of Plant Pathology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA.
Morgan Rebekah
Dahlbeck Douglas
Borsani Omar
Villegas Andy
Zhu Jian-Kang
Staskawicz Brian J
Jin Hailing
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0608258103
Published
2006-11-21
Epub
2006-00-27
Pages
18002-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1693862
Subset
IM
Grants
NIGMS NIH HHS · R01 GM070795 · United States
PHS HHS · R01-FM069680-01 · United States
NIGMS NIH HHS · R01 GM059138 · United States
NIGMS NIH HHS · R01GM59138 · United States
NIGMS NIH HHS · R01GM070795 · United States
External Links
PubMed source DOI: 10.1073/pnas.0608258103 PMC Full Text View journal details
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