Home LiteratureArticle Details
PMID: 17114582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula.

Genes & development ·Vol. 20 ·No. 22 ·2006-11-15 ·Pages 3084-8

Combier JP, Frugier F, de Billy F, Boualem A, El-Yahyaoui F, Moreau S, Vernié T, Ott T, Gamas P, Crespi M, Niebel A

Abstract

In the model legume Medicago truncatula, we identified a new transcription factor of the CCAAT-binding family, MtHAP2-1, for which RNA interference (RNAi) and in situ hybridization experiments indicate a key role during nodule development, possibly by controlling nodule meristem function. We could also show that MtHAP2-1 is regulated by microRNA169, whose overexpression leads to the same nodule developmental block as MtHAP2-1 RNAi constructs. The complementary expression pattern of miR169 and MtHAP2-1 and the phenotype of miR169-resistant MtHAP2-1 nodules strongly suggest, in addition, that the miR169-mediated restriction of MtHAP2-1 expression to the nodule meristematic zone is essential for the differentiation of nodule cells.

MeSH Terms
Base Sequence Gene Expression Regulation, Plant Medicago truncatula/growth & development Meristem/cytology,ultrastructure MicroRNAs/genetics,metabolism Molecular Sequence Data Phenotype Plant Proteins/genetics,metabolism RNA Interference RNA Precursors/metabolism RNA, Messenger/genetics,metabolism Root Nodules, Plant/cytology,growth & development Symbiosis Transcription, Genetic
Chemicals
MicroRNAs Plant Proteins RNA Precursors RNA, Messenger
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Combier Jean-Philippe
LIPM (Laboratoire des Interactions Plantes-Microorganismes) INRA-CNRS (Institut de National de la Recherche-Centre national de la recherche scientifique), 31326 Castanet-Tolosan, France.
Frugier Florian
de Billy Françoise
Boualem Adnane
El-Yahyaoui Fikri
Moreau Sandra
Vernié Tatiana
Ott Thomas
Gamas Pascal
Crespi Martin
Niebel Andreas
References (27)
27 references, click to expand
  1. Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis.
    Plant Physiol. 2006 Jun;141(2):607-19 PMID: 16617092
  2. MicroRNAS and their regulatory roles in plants.
    Annu Rev Plant Biol. 2006;57:19-53 PMID: 16669754
  3. Regulation of novel members of the Arabidopsis thaliana CCAAT-binding nuclear factor Y subunits.
    Gene. 2002 Jan 23;283(1-2):41-8 PMID: 11867211
  4. Ternary complex formation between MADS-box transcription factors and the histone fold protein NF-YB.
    J Biol Chem. 2002 Jul 19;277(29):26429-35 PMID: 11971906
  5. LEAFY COTYLEDON1-LIKE defines a class of regulators essential for embryo development.
    Plant Cell. 2003 Jan;15(1):5-18 PMID: 12509518
  6. Arabidopsis LEAFY COTYLEDON1 represents a functionally specialized subunit of the CCAAT binding transcription factor.
    Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):2152-6 PMID: 12578989
  7. LysM domain receptor kinases regulating rhizobial Nod factor-induced infection.
    Science. 2003 Oct 24;302(5645):630-3 PMID: 12947035
  8. OsHAP3 genes regulate chloroplast biogenesis in rice.
    Plant J. 2003 Nov;36(4):532-40 PMID: 14617083
  9. The B subunit of the CCAAT box binding transcription factor complex (CBF/NF-Y) is essential for early mouse development and cell proliferation.
    Cancer Res. 2003 Dec 1;63(23):8167-72 PMID: 14678971
  10. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA.
    Mol Cell. 2004 Jun 18;14(6):787-99 PMID: 15200956
  11. MicroRNA regulation of the CUC genes is required for boundary size control in Arabidopsis meristems.
    Development. 2004 Sep;131(17):4311-22 PMID: 15294871
  12. Expression profiling in Medicago truncatula identifies more than 750 genes differentially expressed during nodulation, including many potential regulators of the symbiotic program.
    Plant Physiol. 2004 Oct;136(2):3159-76 PMID: 15466239
  13. Correlation between ultrastructural differentiation of bacteroids and nitrogen fixation in alfalfa nodules.
    J Bacteriol. 1990 Aug;172(8):4295-306 PMID: 2376562
  14. Synchronous expression of leghaemoglobin genes in Medicago truncatula during nitrogen-fixing root nodule development and response to exogenously supplied nitrate.
    Plant Mol Biol. 1991 Sep;17(3):335-49 PMID: 1883994
  15. Rhizobium meliloti Nod factors elicit cell-specific transcription of the ENOD12 gene in transgenic alfalfa.
    Plant J. 1994 Aug;6(2):241-9 PMID: 7920714
  16. Rhizobium meliloti lipooligosaccharide nodulation factors: different structural requirements for bacterial entry into target root hair cells and induction of plant symbiotic developmental responses.
    Plant Cell. 1994 Oct;6(10):1357-74 PMID: 7994171
  17. Symbiosis-specific expression of two Medicago truncatula nodulin genes, MtN1 and MtN13, encoding products homologous to plant defense proteins.
    Mol Plant Microbe Interact. 1998 May;11(5):393-403 PMID: 9574507
  18. Role of the CCAAT-binding protein CBF/NF-Y in transcription.
    Trends Biochem Sci. 1998 May;23(5):174-8 PMID: 9612081
  19. Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells.
    Cell. 1998 Jun 26;93(7):1195-205 PMID: 9657152
  20. Nod factor signaling genes and their function in the early stages of Rhizobium infection.
    Curr Opin Plant Biol. 2005 Aug;8(4):346-52 PMID: 15955723
  21. Regulation of Arabidopsis shoot apical meristem and lateral organ formation by microRNA miR166g and its AtHD-ZIP target genes.
    Development. 2005 Aug;132(16):3657-68 PMID: 16033795
  22. Computational detection of microRNAs targeting transcription factor genes in Arabidopsis thaliana.
    Comput Biol Chem. 2005 Oct;29(5):360-7 PMID: 16221572
  23. MtENOD11 gene activation during rhizobial infection and mycorrhizal arbuscule development requires a common AT-rich-containing regulatory sequence.
    Mol Plant Microbe Interact. 2005 Dec;18(12):1269-76 PMID: 16478046
  24. Genetics and functional genomics of legume nodulation.
    Curr Opin Plant Biol. 2006 Apr;9(2):110-21 PMID: 16458572
  25. Characterization of 43 non-protein-coding mRNA genes in Arabidopsis, including the MIR162a-derived transcripts.
    Plant Physiol. 2006 Apr;140(4):1192-204 PMID: 16500993
  26. The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA.
    Plant J. 2006 May;46(3):462-76 PMID: 16623906
  27. Medicago truncatula ENOD11: a novel RPRP-encoding early nodulin gene expressed during mycorrhization in arbuscule-containing cells.
    Mol Plant Microbe Interact. 2001 Jun;14(6):737-48 PMID: 11386369
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.402806
Published
2006-11-15
Pages
3084-8
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1635144
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.402806 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2