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PMID: 12509518 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

LEAFY COTYLEDON1-LIKE defines a class of regulators essential for embryo development.

The Plant cell ·Vol. 15 ·No. 1 ·2003-01-00 ·Pages 5-18

Kwong RW, Bui AQ, Lee H, Kwong LW, Fischer RL, Goldberg RB, Harada JJ

Abstract

Arabidopsis LEAFY COTYLEDON1 (LEC1) is a critical regulator required for normal development during the early and late phases of embryogenesis that is sufficient to induce embryonic development in vegetative cells. LEC1 encodes a HAP3 subunit of the CCAAT binding transcription factor. We show that the 10 Arabidopsis HAP3 (AHAP3) subunits can be divided into two classes based on sequence identity in their central, conserved B domain. LEC1 and its most closely related subunit, LEC1-LIKE (L1L), constitute LEC1-type AHAP3 subunits, whereas the remaining AHAP3 subunits are designated non-LEC1-type. Similar to LEC1, L1L is expressed primarily during seed development. However, suppression of L1L gene expression induced defects in embryo development that differed from those of lec1 mutants, suggesting that LEC1 and L1L play unique roles in embryogenesis. We show that L1L expressed under the control of DNA sequences flanking the LEC1 gene suppressed genetically the lec1 mutation, suggesting that the LEC1-type B domains of L1L and LEC1 are critical for their function in embryogenesis. Our results also suggest that LEC1-type HAP3 subunits arose from a common origin uniquely in plants. Thus, L1L, an essential regulator of embryo development, defines a unique class of plant HAP3 subunits.

MeSH Terms
Amino Acid Sequence Arabidopsis/embryology,genetics,growth & development Arabidopsis Proteins/genetics,metabolism CCAAT-Enhancer-Binding Proteins/genetics,metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Regulator/genetics In Situ Hybridization Molecular Sequence Data Multigene Family/genetics Mutation Phylogeny Plant Proteins/genetics,metabolism RNA, Plant/genetics,metabolism Seeds/genetics,growth & development Sequence Homology, Amino Acid Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins CCAAT-Enhancer-Binding Proteins HAP proteins, Arabidopsis HAP3a protein, Arabidopsis LEC1 protein, Arabidopsis Plant Proteins RNA, Plant Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kwong Raymond W
Section of Plant Biology, Division of Biological Sciences, One Shields Avenue, University of California, Davis, California 95616, USA.
Bui Anhthu Q
Lee Hyeseung
Kwong Linda W
Fischer Robert L
Goldberg Robert B
Harada John J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.006973
Published
2003-01-00
Pages
5-18
Language
English
Region
England
NLM ID
9208688
PMCID
PMC143447
Subset
IM
Databases
GENBANK
AF533650, AY138461
External Links
PubMed source DOI: 10.1105/tpc.006973 PMC Full Text View journal details
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